Trabajos de Titulación - Medicina Veterinaria
URI permanente para esta colección
Examinar
Envíos recientes
- ÍtemAcceso AbiertoEfecto de microorganismos benéficos sobre los niveles de amoniaco y digestibilidad alimenticia en pollos Broiler(Universidad Católica de Cuenca., 2024) Briseño Mejía, Banner Giovanni; Vázquez Vásquez, Jacinto Enrique; 1150598645The current research aimed at evaluating the effect of adding beneficial microorganisms in the drinking water and on the poultry house floor on ammonia levels and feed digestibility parameters in broiler chickens. Six hundred 1-day-old broilers were distributed under a completely randomized design; the treatments to be evaluated were T0 (control) and T1 (0.1% beneficial microorganisms), each with four replicates and 75 experimental units. The variables evaluated were production parameters (weekly weight, feed consumption, weight gain, feed conversion, carcass weight, mortality percentage), feed digestibility (crude protein, organic matter, inorganic matter), and ammonia levels inside the poultry house. The results showed that T1 increased weight gain and improved feed conversion in week 1, feed digestibility was affected in ash and protein, and mortality percentage decreased. However, carcass weight was not affected. Finally, ammonia levels inside the house did not show significant differences between treatments during the research. In conclusion, the addition of beneficial microorganisms at a rate of 0.1% in the drinking water and the broiler poultry house do not have a positive impact on the levels of ammonia inside the poultry house. However, it increases weight gain and bird feed conversion and decreases the percentage of mortality. Keywords: Beneficial microorganisms, production parameters, broiler chicken
- ÍtemAcceso AbiertoEvaluación de la Vitamina K y Coenzima Q10 en la refrigeración de semen equino(Universidad Católica de Cuenca., 2024) Cabrera Saldaña, Juan Carlos; Argudo Garzón, Daniel; 0105709489This study highlights how vitamin K and coenzyme Q10 may influence the quality of cryopreserved sperm in horses, whose viability tends to be lower compared to other species, thereby limiting advancements in reproductive biotechnology. Beginning of the form. Therefore, the justification for this study stems from the need to overcome the limitations of equine fertility. To assess equine semen, the dependent variables included motility and sperm abnormalities, while the independent variables were the presence of vitamin K, coenzyme Q10, and a control group without these supplements. The viability of vitamin K (T2) had a slightly higher mean of 60.38 ± 2.08. Regarding membrane functionality, there were differences between treatments, with T2 (83.04 + 11.03) standing out compared to T1 and T3. On the other hand, after 48 hours of freezing, there is a consistent decrease in sperm values compared to the first 24 hours. Variations in mitochondrial activity were observed between the treatments, indicating a deterioration in sperm quality. The results indicate that sperm can be frozen for 24 hours without significant changes, but extended storage is not recommended. Keywords: Fertility, Coenzyme Q10, Vitamin K, Semen
- ÍtemAcceso AbiertoEvaluación de la coenzima Q10 y la vitamina K en la congelación de semen equino(Universidad Católica de Cuenca., 2024) Pilco Gavilanez, Danny Javier; Argudo Garzón, Daniel; 0606175586Frozen equine semen shows lower viability than other species, mainly due to specific equine factors or individual variability per breeder. Additionally, breeding males are selected for their performance in various equestrian events rather than their reproductive capacity, creating a challenge in preserving and enhancing the animal's genes. This study is justified by estimating that adding coenzyme Q10 and vitamin K to frozen spermatozoa has a beneficial effect on their motility and other seminal quality parameters. For this purpose, variables such as motility, mobility, abnormalities, membrane functionality, and mitochondrial integrity were considered. Independent variables such as fresh sperm, coenzyme Q10 (treatment), and vitamin K (treatment) were used compared to a control group. The results were significant for sperm motility, with 92.20 ± 1.82 for the third horse. Regarding sperm concentration, a peak was shown in equine two with 115.60 ± 17.07, contributing to a total of approximately 1398.19 x 10⁶ ejaculated sperm. In addition, the data suggested no significant variation in the application of coenzyme Q10 and vitamin K regarding mitochondrial motility and mitochondrial activity. This led to the conclusion that adding coenzyme Q10 and vitamin K to sperm does not significantly effect quality parameters such as motility and mitochondrial integrity. Only in certain situations could this improve the quality of equine semen by cryopreservation. Keywords Equine., semen., freezing., coenzyme Q10., vitamin K.
- ÍtemAcceso AbiertoEfectos de los fitoestrógenos de la alfalfa en la ovulación de las cobayas(Universidad Católica de Cuenca., 2024) Párraga Mieles, Andrés Marcelo; Bravo Alvarez, María Ximena; 0104653241This study investigated how phytoestrogens present in the diet affect estrogen levels in guinea pigs. The results presented interesting data on phytoestrogens in the balance of guinea pigs through two treatments and a control. It was concluded that phytoestrogens do not cause a significant decrease in estrogen levels in these small rodents. Phytoestrogens are natural compounds in certain plant foods; it has been speculated that they could affect animal hormone levels. This specific research, however, challenges that aforementioned belief. Blood samples from guinea pigs exposed to different concentrations of phytoestrogens were evaluated, and their estrogen levels were measured, resulting in no significance in the use of exclusive alfalfa diets (T1) and exclusive balanced feed (T2). However, there was significance in the use of mixed feeding (T3). The results suggest that, although there were some differences in the size of secondary follicles between treatments, there were no statistically significant differences in other parameters analyzed, such as the size of primordial follicles and eggs, nor in the correlation between the number of eggs and blood estradiol levels. This has important implications for the breeding and management of these animals, especially in environments where foods rich in phytoestrogens are used. Keywords Guinea pigs; phytoestrogens; alfalfa; estradiol
- ÍtemEmbargoPropóleo como alternativa para el tratamiento de mastitis subclínica bovina en la parroquia Cumbe(Universidad Católica de Cuenca., 2024) Landi Santander, Johanna Patricia; Cuenca Condoy, Mercy del Cisne; 0105326375; Villa, SusanaThe objective of the study was to evaluate the effect of the hydroalcoholic extract of propolis against the bacteria that cause subclinical bovine mastitis. The experiment was run in two phases. In the first phase, the propolis extract was obtained through the maceration method, using 90º alcohol as a solvent. In the second phase, bacteria were isolated and identified from raw milk samples from the Cumbe parish using the MALDI-TOF technique. Antibiogram disks impregnated with different concentrations of propolis were used to determine bacterial susceptibility. Statistical analysis used the Kruskal-Wallis test due to non-normality of the data. Eight bacteria were identified, including Staphylococcus aureus and Streptococcus agalactiae as contagious microorganisms, and environmental type Pantoea agglomerans, Staphylococcus warneri, Staphylococcus epidermidis, Staphylococcus haemolyticus, Arthrobacter gandavensis and Kluyvera cryocrescens were reported. The results showed widespread resistance to the antibiotics penicillin and doxycycline. However, Staphylococcus aureus, Staphylococcus warneri and Arthrobacter gandavensis showed susceptibility to propolis, with significant inhibition zones starting at 0.25 mL. The minimum inhibitory concentration (MIC) of propolis against S. aureus and S. agalactiae was determined at doses of 0.1 mL and 0.05 mL, respectively. The findings suggest that propolis could be an effective therapeutic alternative for the treatment of subclinical bovine mastitis, especially against certain pathogens resistant to conventional antibiotics, highlighting the urgent need to investigate new therapeutic options for this disease. Key words: Bovine mastitis; etiology; propolis
