Trabajos de Titulación - Medicina Veterinaria
URI permanente para esta colecciónhttps://dspace.ucacue.edu.ec/handle/ucacue/53
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Ítem Acceso Abierto Presencia de Cryptococcus neoformans en balanceado a granel para perros en cuatro mercados de la ciudad de Cuenca.(Universidad Católica de Cuenca., 2025) Morales Bucheli , Daniel Fernando; Campos Murillo, Nathalie del Consuelo; 0107220030Cryptococcus neoformans is an encapsulated yeast of medical and veterinary importance, associated with cryptococcosis ―an opportunistic mycosis that mainly affects immunocompromised individuals. This study aimed to evaluate the presence of Cryptoccocus neoformans in bulk dog food sold in five busy markets in the city of Cuenca, considering this food as a possible vehicle for dissemination of the fungus to pets and people. A total of 45 samples were collected cultured on Sabouraud agar supplemented with Chloramphenicol, and incubated at 37°C for seven days. The macroscopic study showed the growth of whitish and creamy colonies accompanied by opportunistic molds. By microscopic examination and subsequent identification by MALDI-TOF mass spectrometry, the presence of Candida parapsilosis was confirmed. C. neoformans was not detected in the samples analyzed. These findings show the microbiological contamination of food by yeasts associated with pigeon droppings, which represents a potential risk to public health, especially for vulnerable populations exposed to contaminated aerosols. Keywords: Cryptococcus neoformans, dog food, microbiological contamination, pigeon droppings, public health.Ítem Acceso Abierto Simbióticos reforzados con enzimas: una alternativa sostenible a los antibióticos promotores de crecimiento en pollos Broiler(Universidad Católica de Cuenca., 2025) Andrade Muyulema , Erick Saul; Ramirez Gallardo, Bismarck Ney; Cuenca Condoy, Mercy del Cisne; 0302330196; 0705109957This study evaluated the effect of dietary supplementation with enzyme-enhanced symbiotic on productive, immunological, digestive, and histo-morphological parameters in broiler chickens. The research was conducted under commercial conditions in the Balsas canton, El Oro province (Ecuador), using a longitudinal experimental design. A total of 84,000 birds were randomly allocated to four treatments: T0 (control), T1 (0.01%), T2 (0.03%), and T3 (0.05%) symbiotic, all combined with 0.01% protease enzymes. Productive performance, apparent digestibility, intestinal morphometry, and serum immunoglobulins (IgY, IgM, IgA) were assessed on days 1 and 21 of age. Treatment T2 (0.03%) yielded the best zootechnical performance, with greater weight gain, more efficient feed conversion, and enhanced intestinal mucosal development, as evidenced by increased villus length and density. Additionally, T2 improved crude protein and fat digestibility and significantly stimulated IgM production, suggesting more effective enteric immune activation. In contrast, T3 (0.05%) exhibited negative effects on intestinal morphology and weight gain, indicating that higher concentrations may cause physiological interference. No clear dose-dependent trend was observed for crude fiber and dry matter digestibility. Statistical analysis confirmed significant differences (p < 0.05) among treatments. In conclusion, supplementation with 0.03% symbiotic enhanced with enzymes proved to be the most effective strategy for improving intestinal health, nutrient utilization, and productive performance in broiler chickens, establishing itself as a promising functional alternative for intensive poultry systems. Keywords: Synbiotics; enzymes; broiler chickens; productive parameters; intestinal morphometry; immunoglobulins; digestibilit.Ítem Acceso Abierto Monitorización hemodinámica en caballos temperamentales mediante el uso de acepromacina a dosis bajas por vía subcutánea(Universidad Católica de Cuenca., 2025) Brito Duran, Bryan Fernando; Landi Torres, Edwin Ernesto; 0106390479; Castillo Zhizpon, Sandra ElizabethAcepromazine (ACE) is a phenothiazine tranquilizer that acts as a dopamine antagonist. The objective of this study was to evaluate the degree of sedation along with the collection of physiological parameters (diastolic pressure, systolic pressure, temperature, and oxygen saturation). Three doses (0.02 mg/kg, 0.04 mg/kg, and 0.06 mg/kg) were administered subcutaneously to euphoric horses in order to determine which of them provides a sedative effect with minimal adverse reactions. The research was conducted in the parish of San Pablo de Shaglli, part of the Santa Isabel Canton, Azuay Province, located at an altitude of 4160 meters above sea level. It is important to note that these conditions impose physiological differences, particularly in oxygen saturation and cardiovascular response. The study involved 18 Creole horses, with one repetition per dose, totaling 54 repetitions. Physiological parameters were recorded before and after ACE administration, with behavioral evaluation performed 20 minutes post-application. The results showed that the ideal dose is 0.04 mg/kg, as it generated a favorable response, proving to be safer and more effective compared to the other doses, especially considering the geographical conditions of the area. The 0.02 mg/kg dose was insufficient, as it produced little to no sedation. On the other hand, the 0.06 mg/kg dose caused excessive sedation, evidenced mainly by a marked and risky drop in blood pressure, which is unfavorable given the environmental conditions. Keywords: acepromazine, equines, altitude, physiological constantsÍtem Acceso Abierto Potencial terapéutico del extracto liofilizado de alcachofa en el tratamiento del barro biliar en perros: Estudio de casos(Universidad Católica de Cuenca., 2025) Palaguachi Pizarro, Cristopher Xavier; Iñiguez Heredia, Franklin Alfredo; 0107083602Biliary sludge is a common hepatobiliary condition in canines, characterized by the accumulation of echogenic sediment within the gallbladder, and is associated with the risk of developing cholelithiasis and other liver complications. In response to the need for alternative therapies, this study evaluated the therapeutic potential of lyophilized extract of Cynara scolymus (artichoke) for the treatment of biliary sludge in dogs. A case study was conducted with two dogs diagnosed with type II biliary sludge, which received artichoke extract (20 mg/kg every 12 hours) for 60 days. The treatment was monitored through sequential ultrasound evaluations and liver biochemical analyses performed before, during, and after the protocol. Results showed a progressive reduction of echogenic material in the gallbladder, transitioning from type II to type I sludge by the end of the treatment. A decrease in cholestatic markers such as alkaline phosphatase and total and direct bilirubin was observed, along with stable values for total proteins and cholesterol. Although the small sample size limits broader conclusions, the findings suggest hepatoprotective and choleretic effects of the artichoke extract. It is concluded that lyophilized Cynara scolymus extract represents a promising phytotherapeutic alternative for managing biliary sludge in veterinary medicine, supporting further studies with controlled designs and larger populations. Keywords: biliary sludge, cynara scolymus, veterinary medicine, hepatoprotection, phytotherapyÍtem Acceso Abierto Evaluación del comportamiento alimentario de Holcosus orcesi mantenida bajo cuidado humano dentro del Bioparque AMARU(Universidad Católica de Cuenca., 2025) Saldaña Pillco , Adriana Carolina; Alvarado Alvarado , Juan Carlos; 0107958928The Orcés’ Blue Whiptail (Holcosus orcesi) is a species endemic to the Santa Isabel canton, Azuay province, Ecuador. It is distinguished from other specimens by the conformation of its scales and its coloring, which allows its differentiation. It is categorized as critically endangered according to the International Union for Conservation of Nature (IUCN). This is a Sine Qua Non condition to protect it in conservation centers. To this end, the taxonomic and entomological identification of around 12 specimens collected in their natural habitat was conducted to recognize the diversity present in their environment. Additionally, a coproparasite analysis was performed to understand their feeding strategy, examining part of their diet and the presence of possible parasites. Finally, a species of cricket (Acheta domesticus) was selected, which underwent a bromatological analysis to develop a base diet in order to improve health and stimulate the natural behavior of this species. At the same time, their daily activity was recorded through an ethogram with 20 behavioral patterns related to daily maintenance and eating behaviors. A decrease in negative behaviors could be observed (p=0.063) and also a significant variation in behaviors related to daily maintenance (p=0.021), which suggests that the appearance of new behaviors may be related to internal and external factors of environmental and food enrichment; something that occurred within their microhabitat. Therefore, it is necessary to monitor this species to guarantee its survival and conservation. Keywords: behavioral patterns, IUCN, conservation, Azuay, endemic.Ítem Acceso Abierto Presencia de fasciola hepática en bovinos de las riberas del río Yanuncay en la parroquia San Joaquín(Universidad Católica de Cuenca., 2025) Sarate Landi, Edisson Xavier; Ulloa Nivelo, Catherine Rosario; 0107318248Fasciola hepatica, or Liver Fluke, is a trematode parasite that affects public health as it is a zoonotic disease resulting in economic losses in livestock farming and health risks in the population. This study aimed to analyze the presence of Fasciola hepatica in bovine feces from animals grazing on the banks of the Yanuncay River in the San Joaquín parish, Cuenca-Azuay. For this purpose, 128 fecal samples were evaluated from animals from 23 farms, which were collected directly from the animals' rectum. Ninety-three females and 35 males were recorded. The analysis was performed using the simple sedimentation method, resulting in the absence of Fasciola, however, additional findings were reported, such as the presence of Coccidia (0.80%), Amoebas (0.63%), and Balantidium (0.01%), predominating in calves and adults, with mild severity. In addition, a comparison of the results was made with other previous regional studies, where none reported a prevalence greater than 37%. It is proposed that the absence of Fasciola hepatica is related to environmental conditions such as the drought that affected the region in the previous months, whereby the lack of humidity decreased the population of intermediate snails. Therefore, it is essential to carry out more exhaustive epidemiological investigations in the livestock sector to understand the dynamics of infection in greater depth and develop effective control strategies, as well as continuing to monitor sanitary and environmental conditions for future epidemics, guaranteeing public and food health. Keywords Fasciola hepatica, Presence, Simple Sedimentation, Post-droughtÍtem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Cabrera Cochancela, Catalina Alexandra; Ortiz Alvarado, Maira Briscila; 0105400220Ítem Acceso Abierto Administración de CBD para el manejo de desórdenes de ansiedad en gatos(Universidad Católica de Cuenca., 2025) Molina Bernal , Brittney Lissette; Castillo Hidalgo, Edy Paul; 0107408635The research work focuses on the therapeutic management of anxiety in cats, a topic that generates a growing concern in veterinary medicine due to the high sensitivity of this species to stressors. In this context, the use of cannabidiol (CBD), a non-psychotropic component of cannabis, is explored as a possible therapeutic alternative to reduce symptoms and signs of anxiety in felines. According to a study conducted by (Mentzel et al., 2022), the following prevalence of anxiety disorders has been observed in cats: generalized anxiety disorder (15%), which is characterized by unjustified anxiety; disocialization (23%), which is defined as the absence of social behavior; deterritorialization (14%), which is shown as an alteration of territorial behavior; phobia (1.4%), understood as excessive fear; compulsive disorder (4%), which manifests itself in stereotyped behaviors; dissociative disorder (2%), which includes self-harm; and intermittent explosive aggression (3%), characterized by the presentation of unpredictable aggression. The aim of the present research was to evaluate the efficacy and safety of administering different doses of cannabidiol (CBD) compared to a control group, to determine its effects in cats clinically diagnosed with anxiety disorders. To meet this objective, nine domestic cats (Felis catus) with a clinical diagnosis of anxiety were selected. An experimental design of staggered doses was applied: 0.5 mg/kg and 1.0 mg/kg of CBD, plus a control group that received a placebo (0.9% saline). The observations were carried out for four weeks in rooms equipped to minimize external variables and to be able to objectively record the behavioral parameters that make up the FAS (Fear, anxiety, and stress) scale. Cats receiving 0.5 mg/kg showed a significant reduction in signs and symptoms of anxiety with a low incidence of side effects. In contrast, patients who received a dose of 1.0 mg/kg presented variable responses with a slight increase in side reactions. Compared to the control group, the treated animals showed greater emotional stability, reflecting the potential usefulness of CBD as an alternative treatment for anxiety disorders. Therefore, CBD, and in particular its administration orally at doses of 0.5 mg/kg, is a safe and effective option to reduce the signs of anxiety in felines. However, it is recommended to expand the research with a larger number of patients and longer follow-up periods to confirm these findings and promote their use in clinical practice. Keywords: CBD; cats; anxiety; cannabidiol; FAS scaleÍtem Acceso Abierto Efecto biocida del extracto de raíz del barbasco (lonchocarpus utilis)en el control de piojos en cuyes(Universidad Católica de Cuenca., 2025) Calle Encalada, Juan David; Cuenca Condoy, Mercy; 0302606504The study evaluated the biocidal effect of Cubé resin (Lonchocarpus utilis) for controlling lice in guinea pigs. A total of 180 improved-genotype guinea pigs in the growth phase (400 ± 50 g) were used, distributed across six treatments: T0 (control), T1 (25%), T2 (20%), T3 (15%), T4 (10%), and T5 (5%), with three replicates of 10 guinea pigs each. Cubé resin underwent collection, cleaning, drying, crushing, pulverization, and sieving. Its quality and toxicity were assessed through a bioassay with Artemia salina, determining an LC50 of 0.50% at three hours. The biocidal efficacy was tested in two phases. In the in vitro phase, 150 lice were exposed to five concentrations (T1, 100%; T2, 80%; T3, 60%; T4, 40%; and T5, 20%), with 10 lice per concentration. In the in vivo phase, infested guinea pigs were treated with Cubé resin at 25%, 20%, 15%, 10%, and 5% at a dose of 1% of their live weight, and lice mortality was evaluated at 6, 12, and 18 hours. The in vitro results showed significant differences (p<0.05), achieving the highest mortality (100%) at the 100% concentration after 18 hours. In the in vivo phase, all concentrations reached 100% mortality at 6 hours, whereas the commercial product achieved its maximum mortality at 12 hours. It is concluded that Cubé resin at concentrations below 25% has a biocidal effect and could serve as an alternative to commercial treatments. Keywords Lonchocarpus utilis, Barbasco, Lice, Guinea pigs..Ítem Acceso Abierto Efecto de la concentración de la L-carnitina en congelación del semen ovino(Universidad Católica de Cuenca., 2025) Valdez Pinoargote, Girabel Gissela; Moscoso Piedra, Andrés Leonardo; 0804298040Sperm cryopreservation is a key technique in animal reproduction, particularly in ruminants, enabling assisted reproduction and benefiting veterinary medicine. L-Carnitine, an amino acid derived from lysine and methionine, plays a crucial role in preserving membrane integrity and mitochondrial function. The aim of this study was to assess the effect of Lcarnitine supplementation at doses of 1M, 0.750mM, 0.500mM, and 0.100mM on the sperm quality of three sheep breeds (Dorper, Kathadin, and Pelibuey). Semen was collected from each sheep over multiple mounts, resulting in three ejaculates per animal, with a total of nine samples, and a control test with semen free of L-carnitine. These samples were evaluated through laboratory tests (Eosin-Nigrosin, Propidium Iodide, and Rodamine) and analyzed using the CASA system to measure parameters such as PR, NP, IM, VCL, VAP, VSL, STR, LIN, ALH, and BCF. The results favored the 1M dose (highest concentration) across permeability, viability, and kinetics tests. Statistical differences were found in the LIN and STR values, consistently favoring the 1M concentration compared to the control. Keywords: Cryopreservation; L-Carnitine; Sheep; SpermatozoaÍtem Acceso Abierto Resistencia y Acritud de Pasteurella multocida en gatos domésticos (felis catus) en cuatro clínicas veterinarias del sur de Cuenca(Universidad Católica de Cuenca., 2025) Tinoco Suscal, Mateo Fabián; Aguilar Caivinagua, Santiago Andrés; 0105993687Pasteurella multocida is a zoonotic pathogen of high relevance in public health; however, information on its antimicrobial resistance (AMR) profiles and virulence factors in domestic cats is limited. This study aims to contribute to understanding these critical aspects, which are fundamental for developing effective strategies to prevent and control zoonoses. This study evaluates the AMR and virulence factors of P. multocida isolated from the oral mucosa of Felis catus in four veterinary clinics in southern Cuenca. Fifty oral mucosa samples were collected using sterile swabs, and susceptibility to 10 commonly used antimicrobials in veterinary medicine was analyzed. MALDI-TOF mass spectrometry was used to confirm the specific presence of Pasteurella multocida and bacterial load. The results will reveal the AMR profiles and virulence factors of the isolated strains, evaluating their potential zoonotic impact and implications for public health. This research will significantly contribute to the knowledge of the epidemiology of P. multocida in felines, identifying effective antimicrobials, those with resistance, and the present virulence factors. This research will significantly contribute to the knowledge of the epidemiology of Pasteurella multocida in Felis catus populations, identifying antimicrobials with therapeutic efficacy and those for which resistance is evident, as well as virulence factors. Keywords: Bacteria; Zoonosis; Antimicrobial Agents; Oral Mucosa; Virulence; Public Health; Mass Spectrometry; AMR; Felis Catus; Veterinary MedicineÍtem Embargo Evaluación de la resistencia antimicrobiana en cepas de staphylococcus aureus aisladas de la mucosa en un gato de las pampas (Leopardus garleppi)(Universidad Católica de Cuenca., 2025) Tinizhañay Chicaiza, Aracely Pilar; Maldonado Cornejo, Manuel Esteban; 0302934534.Ítem Embargo Evaluación de los parámetros productivos, control de temperatura y niveles de amoníaco en una producción avícola con cama nueva y cama reutilizada(Universidad Católica de Cuenca., 2025) Valarezo Ramirez, Hender Isaac; Cuenca Condoy, Mercy del Cisne; 0706104999.Ítem Embargo Respuesta superovulatoria de un protocolo tradicional con remplazo parcial de pluset (FSH-LH) por una dosis de eCG en bovinos(Universidad Católica de Cuenca., 2025) Suárez Ortiz, María Julissa; Alvarado Alvarado, Juan Carlos; 0350097101.Ítem Embargo Efecto biocida del Barbasco (Lonchocarpus utilis) en el control de garrapatas (Rhipicephalus Boophilus microplus)(Universidad Católica de Cuenca., 2025) Torres Lozano, Oscar Lennin; Cuenca Condoy, Mercy del Cisne; 1400715551Ítem Acceso Abierto Tiempo de infiltración de los huevos de toxocara canis en función del tipo de suelo(Universidad Católica de Cuenca., 2025) Villa Lucero, Marcia Catalina; Quinteros Rodas, Wilson Olmedo; 0104760509A study was carried out to determine the level of infiltration of Toxocara canis spp. eggs in three types of soil, as a contamination factor in soils or green areas, through an experiment that artificially simulated the parasite’s mobility in the laboratory. Eggs from the specimen were identified in fecal samples, and refined sawdust was used as a biosimulator material, which were evaluated together using the Faust Method. The three types of soil —sand, humus, and common soil (silt loam)— were placed in a cylindrical PVC tube measuring 12.7 cm (5 inches) x 20 cm in height, where the material was deposited. For 10 days, the samples were evaluated at three depths (5 cm, 10 cm, and 15 cm) to determine the degree of infiltration of Toxocara canis eggs. The results show positive cases for each type of soil, regardless of the soil type, with a higher number of positive cases at a depth of 5 cm. The presence and absence of simulant particles smaller than 100 um were evaluated, and finally, Toxocara eggs were identified at a depth of 5 cm. It is concluded that soils supersaturated with water inhibit infiltration, with higher infiltration rates observed after the first irrigation due to natural runoff. This suggests that the parasite could survive at these levels without exposure to sunlight, making this a preliminary study to understand the mobility of Toxocara canis in different soil types and thus preserve public health. Keywords Biosimulator, Faust Method, Humus, Public Health.Ítem Embargo Efecto de la menaquinona 4 y coenzima Q10 en la calidad espermática de semen porcino(Universidad Católica de Cuenca., 2025) Tapia Benavides , Freddy Josué; Alvarado Alvarado, Juan Carlos; 0107159311.Ítem Embargo Variación de PH y pérdidas por goteo en la premaduración cortes bovinos procedentes del centro de faenamiento del cantón Cañar(Universidad Católica de Cuenca., 2025) Suárez Vásquez, Susana Carolina; Maldonado Cornejo, Manuel Esteban; 0302856745.Ítem Acceso Abierto Estudio retrospectivo de pacientes caninos con pancreatitis aguda (cPL anormal)y su correlación con cambios en la biometría y química sanguínea(Universidad Católica de Cuenca., 2025) Bravo Chimbo, Jennifer Michelle; Aguilar Caivinagua, Jennifer Michelle; 0107432031Acute pancreatitis (AP) is a common disease in dogs, with variable severity and a significant mortality rate ranging from 27% to 58% of cases. Secondary complications, such as azotemia, are a major cause of morbidity and mortality in these patients. This study aimed to analyze the relationship between AP cases in dogs and changes in hematologic and blood chemistry parameters. A retrospective study was conducted at the Austrovet veterinary clinic, where data were collected from 33 medical records of canine patients diagnosed with AP using the canine pancreatic lipase (cPL) test between 2022 and 2023. The prevalence of azotemia and its correlation with hematologic and blood chemistry parameters were determined. The results of this study showed that red blood cell count, hematocrit, and hemoglobin levels were below normal values. Non-regenerative anemia was the most prevalent hematologic disorder, followed by neutrophilia and leukocytosis, suggesting an active systemic inflammatory response. Regarding blood chemistry, azotemia was frequently observed in 36.36% of cases, which could indicate renal impairment. Elevated alkaline phosphatase (39.39%) and serum amylase levels exceeding three times the normal range (39.39%) were other common findings, suggesting the presence of cholangitis and/or pancreatic damage. Thus, these results highlight the importance of hematology and blood chemistry in the early diagnosis of AP, as these abnormalities indicate severe complications such as azotemia. Timely diagnosis and appropriate treatment could help reduce mortality and improve the prognosis of dogs with acute pancreatitis. Keywords: Acute pancreatitis; Azotemia; Hematology; Blood chemistryÍtem Embargo Microdosis de coenzima Q10 como potencializador de la calidad espermática de semen ovino crioconservado(Universidad Católica de Cuenca., 2025) Quinche Guazhambo , Mónica Daniela; Moscoso Piedra, Andrés Leonardo; 0106979446.
