Trabajos de Titulación - Medicina Veterinaria
URI permanente para esta colección
Examinar
Envíos recientes
- ÍtemAcceso AbiertoEvaluación de la Vitamina K y Coenzima Q10 en la refrigeración de semen equino(Universidad Católica de Cuenca., 2024) Cabrera Saldaña, Juan Carlos; Argudo Garzón, Daniel; 0105709489This study highlights how vitamin K and coenzyme Q10 may influence the quality of cryopreserved sperm in horses, whose viability tends to be lower compared to other species, thereby limiting advancements in reproductive biotechnology. Beginning of the form. Therefore, the justification for this study stems from the need to overcome the limitations of equine fertility. To assess equine semen, the dependent variables included motility and sperm abnormalities, while the independent variables were the presence of vitamin K, coenzyme Q10, and a control group without these supplements. The viability of vitamin K (T2) had a slightly higher mean of 60.38 ± 2.08. Regarding membrane functionality, there were differences between treatments, with T2 (83.04 + 11.03) standing out compared to T1 and T3. On the other hand, after 48 hours of freezing, there is a consistent decrease in sperm values compared to the first 24 hours. Variations in mitochondrial activity were observed between the treatments, indicating a deterioration in sperm quality. The results indicate that sperm can be frozen for 24 hours without significant changes, but extended storage is not recommended. Keywords: Fertility, Coenzyme Q10, Vitamin K, Semen
- ÍtemAcceso AbiertoEvaluación de la coenzima Q10 y la vitamina K en la congelación de semen equino(Universidad Católica de Cuenca., 2024) Pilco Gavilanez, Danny Javier; Argudo Garzón, Daniel; 0606175586Frozen equine semen shows lower viability than other species, mainly due to specific equine factors or individual variability per breeder. Additionally, breeding males are selected for their performance in various equestrian events rather than their reproductive capacity, creating a challenge in preserving and enhancing the animal's genes. This study is justified by estimating that adding coenzyme Q10 and vitamin K to frozen spermatozoa has a beneficial effect on their motility and other seminal quality parameters. For this purpose, variables such as motility, mobility, abnormalities, membrane functionality, and mitochondrial integrity were considered. Independent variables such as fresh sperm, coenzyme Q10 (treatment), and vitamin K (treatment) were used compared to a control group. The results were significant for sperm motility, with 92.20 ± 1.82 for the third horse. Regarding sperm concentration, a peak was shown in equine two with 115.60 ± 17.07, contributing to a total of approximately 1398.19 x 10⁶ ejaculated sperm. In addition, the data suggested no significant variation in the application of coenzyme Q10 and vitamin K regarding mitochondrial motility and mitochondrial activity. This led to the conclusion that adding coenzyme Q10 and vitamin K to sperm does not significantly effect quality parameters such as motility and mitochondrial integrity. Only in certain situations could this improve the quality of equine semen by cryopreservation. Keywords Equine., semen., freezing., coenzyme Q10., vitamin K.
- ÍtemAcceso AbiertoEfectos de los fitoestrógenos de la alfalfa en la ovulación de las cobayas(Universidad Católica de Cuenca., 2024) Párraga Mieles, Andrés Marcelo; Bravo Alvarez, María Ximena; 0104653241This study investigated how phytoestrogens present in the diet affect estrogen levels in guinea pigs. The results presented interesting data on phytoestrogens in the balance of guinea pigs through two treatments and a control. It was concluded that phytoestrogens do not cause a significant decrease in estrogen levels in these small rodents. Phytoestrogens are natural compounds in certain plant foods; it has been speculated that they could affect animal hormone levels. This specific research, however, challenges that aforementioned belief. Blood samples from guinea pigs exposed to different concentrations of phytoestrogens were evaluated, and their estrogen levels were measured, resulting in no significance in the use of exclusive alfalfa diets (T1) and exclusive balanced feed (T2). However, there was significance in the use of mixed feeding (T3). The results suggest that, although there were some differences in the size of secondary follicles between treatments, there were no statistically significant differences in other parameters analyzed, such as the size of primordial follicles and eggs, nor in the correlation between the number of eggs and blood estradiol levels. This has important implications for the breeding and management of these animals, especially in environments where foods rich in phytoestrogens are used. Keywords Guinea pigs; phytoestrogens; alfalfa; estradiol
- ÍtemEmbargoPropóleo como alternativa para el tratamiento de mastitis subclínica bovina en la parroquia Cumbe(Universidad Católica de Cuenca., 2024) Landi Santander, Johanna Patricia; Cuenca Condoy, Mercy del Cisne; 0105326375; Villa, SusanaThe objective of the study was to evaluate the effect of the hydroalcoholic extract of propolis against the bacteria that cause subclinical bovine mastitis. The experiment was run in two phases. In the first phase, the propolis extract was obtained through the maceration method, using 90º alcohol as a solvent. In the second phase, bacteria were isolated and identified from raw milk samples from the Cumbe parish using the MALDI-TOF technique. Antibiogram disks impregnated with different concentrations of propolis were used to determine bacterial susceptibility. Statistical analysis used the Kruskal-Wallis test due to non-normality of the data. Eight bacteria were identified, including Staphylococcus aureus and Streptococcus agalactiae as contagious microorganisms, and environmental type Pantoea agglomerans, Staphylococcus warneri, Staphylococcus epidermidis, Staphylococcus haemolyticus, Arthrobacter gandavensis and Kluyvera cryocrescens were reported. The results showed widespread resistance to the antibiotics penicillin and doxycycline. However, Staphylococcus aureus, Staphylococcus warneri and Arthrobacter gandavensis showed susceptibility to propolis, with significant inhibition zones starting at 0.25 mL. The minimum inhibitory concentration (MIC) of propolis against S. aureus and S. agalactiae was determined at doses of 0.1 mL and 0.05 mL, respectively. The findings suggest that propolis could be an effective therapeutic alternative for the treatment of subclinical bovine mastitis, especially against certain pathogens resistant to conventional antibiotics, highlighting the urgent need to investigate new therapeutic options for this disease. Key words: Bovine mastitis; etiology; propolis
- ÍtemAcceso AbiertoTermografía Infrarroja como técnica alternativa para la detección de Mastitis Subclínica Bovina en la parroquia Cum(Universidad Católica de Cuenca., 2024) Vega Cárdenas, Lisseth Estefanía; Cuenca Condoy, Mercy del Cisne; 0105757512The study aimed to validate the use of infrared thermography (IRT) as a diagnostic technique for subclinical bovine mastitis (SBM). A total of 250 Holstein Friesian and crossbred female bovines were evaluated from the first to sixth calving, between 30 and 150 days of lactation, managed under a semi-intensive system in the Cumbe parish, Cuenca-Ecuador. Initially, SBM was detected using infrared thermography, with predefined temperature ranges for healthy and diseased quarters. These thermal ranges were validated through the California Mastitis Test (CMT) field test. From the positive mammary quarters for CMT and IRT, milk samples were collected in 10 ml falcon tubes for somatic cell count (SCC) using Ekomilk Scan. The evaluated variables included Sensitivity, Specificity, Positive Predictive Value (PPV), Negative Predictive Value (NPV), Validity Index, Youden Index, Positive and Negative Likelihood Ratio. The results showed a prevalence of SBM of 28.8% and 16.5% at cow and mammary quarter levels, respectively. Regarding the variables studied, Sensitivity, Specificity, Positive Predictive Value (PPV), Negative Predictive Value (NPV), Accuracy, Youden's Index, Positive Likelihood Ratio, and Negative Likelihood Ratio were determined as 99.46%, 92.19%, 97.60%, 97.37%, 98.33%, 0.92%, 12.73%, and 0.01%, respectively. No positive correlation was found between temperature and environmental humidity with the thermal values of IRT. It is concluded that Infrared Thermography could be implemented as a promising technique for SBM detection. Keywords. Bovine mastitis; infrared thermography; diagnostic technique
