Trabajos de Titulación - Medicina Veterinaria
URI permanente para esta colecciónhttps://dspace.ucacue.edu.ec/handle/ucacue/53
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Item type: Ítem , Access status: Acceso Abierto , Maduración in-vitro de ovocitos bovinos en cultivo de células oviductales(Universidad Católica de Cuenca., 2026) Mora Rojas , Kelly YajairaIn vitro maturation (IVM) of bovine oocytes in culture systems represents a promising strategy for improving meiotic competence and embryonic development in assisted reproduction. This study aimed to evaluate the effect of culturing bovine cumulus-oocyte complexes (COCs) with a monolayer of bovine oviduct epithelial cells cultured in TCM-19 medium. The COCs were classified according to morphological criteria established by the IETS into grades 1 to 4 and assigned to two experimental groups: a control group and a group with oviduct epithelial cells. The degree of cumulus expansion was evaluated on a scale of 0–4, together with the nuclear maturation rate (MII). The data were analyzed using a chi-square test (p > 0.005). The results showed that the control group exhibited a greater degree of cumulus expansion compared to the oviductal cell group, with a statistically significant difference (χ² = 8.00; df = 3; p-value = 0.046). In contrast, no significant difference (p < 0.005) was observed in the nuclear maturation rate between the two treatments, indicating a functional dissociation between cumulus expansion and the resumption of meiosis in the oocyte. The results of this study underscore that the supportive capacity of a monolayer of oviduct epithelial cells for oocytes depends on the functional status and prior hormonal conditioning of the oviductal cells, and that the physical presence of the monolayer alone is not sufficient to ensure complete cumulus expansion during in vitro oocyte maturation. Keywords. Oocytes, epithelial cells, maturation, in vitro, oviduct.Item type: Ítem , Access status: Acceso Abierto , Comparación de la efectividad del uso de yema de huevo de ganso y gallina en la criopreservacion de semen ovino(Universidad Católica de Cuenca., 2026) Andrade Paredes, Andrés PatricioAbstract This study compared the effectiveness of goose and chicken egg yolk in the cryopreservation of ovine semen. Cryopreservation is a crucial process in reproductive biotechnologies; however, itmay cause damage to the sperm membrane due to thermal, oxidative, and osmotic stress. The research was based on the evaluation of ram semen exposed to two Triladyl-based extenders: chicken egg yolk (control group) and goose egg yolk (experimental group), both included at a concentration of 20% in the extender solution. Semen samples were cryopreserved and subsequently assessed for viability (eosin-nigrosin staining), membrane integrity (HOST), total motility, progressive motility, and sperm kinematic parameters using a Computer-Assisted Sperm Analysis (CASA) system. The results showed that most of the evaluated parameters did not differ significantly between treatments. Significant differences were observed in straight-line velocity (VSL) (P ≤ 0.05) and amplitude of lateral head displacement (ALH), with higher values recorded in semen cryopreserved with chicken egg yolk. These findings suggest that goose egg yolk may serve as an equivalent, although not superior, alternative to chicken egg yolk as a semen extender for ovine cryopreservation. Keywords: Cryopreservation; Egg Yolk Extenders; Ram Semen; Sperm Kinetics; Sperm Motility; Membrane Integrity; Assisted Reproduction.Item type: Ítem , Access status: Acceso Abierto , Evaluación longitudinal de cráneo en caninos braquicéfalos sin pedigree mediante la técnica de radiografía(Universidad Católica de Cuenca, 2026) Mendoza Parra , Carla MadelaineThe primary objective of this study was to analyze the cranial morphological changes in non-pedigreed brachycephalic dogs and to identify the incidence of respiratory disorders. Consequently, this study was conducted using a longitudinal approach, with a census of 30 canine patients older than one year, including both males and females. An imaging assessment was performed using two radiographic views: laterolateral and craniodorsoventral. Furthermore, the investigation focused on morphometric measurements obtained with Fiji software, enabling evaluation of cranial morphometric characteristics and their changes over time. Additionally, a tracheal morphometric analysis was performed in some patients to assess possible morphological abnormalities associated with respiratory collapse. Finally, the study aimed to contribute to clinical diagnosis by identifying the early respiratory conditions affecting these patients, such as brachycephalic obstructive airway syndrome (BOAS). Keywords Brachycephalic dogs; cranial morphology; radiography; longitudinal evaluation; respiratory system; tracheal morphometry.Item type: Ítem , Access status: Embargo , EMBARGADO POR MOTIVOS DE INVESTIGACIÓN(Universidad Católica de Cuenca., 2026) Carrillo Salinas, María Daniela...Item type: Ítem , Access status: Embargo , EMBARGADO POR MOTIVOS DE INVESTIGACIÓN(Universidad Católica de Cuenca., 2026) Zhunio Uyaguari, Shenser Xavier...Item type: Ítem , Access status: Acceso Abierto , Programa de adiestramiento de caninos mestizos como soporte emocional para personas en la ciudad de Cuenca(Universidad Católica de Cuenca, 2026) Castillo Agurto , Jhuleidy MaribelThis study evaluated a pilot positive reinforcement training program applied to four adult mixed-breed dogs in the city of Cuenca, Ecuador, with the aim of analyzing their behavioral response, learning capacity, and adaptation to stressful stimuli. A descriptive quasi- experimental design with a mixed-methods approach and case study methodology was used. Individualized training employed the Luring technique and basic commands such as "sit," "heel," and "stay." The evaluation included an adaptation of the Campbell test and a Likert- type scale to assess behavioral response. The results showed improvements in three of the four dogs, demonstrating greater tolerance to stressful stimuli, emotional stability, obedience, and learning. The "stay" command required more time to consolidate. The study concludes that positive reinforcement strengthened the human-animal bond and demonstrates the potential of mixed-breed dogs as emotional support animals, with prior experiences also influencing learning speed. Keywords Training; behavior; potential; adaptation; learning; bonding.Item type: Ítem , Access status: Acceso Abierto , TRABAJO DE TITULACIÓN EMBARGADO CON FINES DE PUBLICACION(Universidad Católica de Cuenca., 2026) Narváez Espinoza, Mayerly Fernanda...Item type: Ítem , Access status: Embargo , Proyecto de Titulación embargado con fines de publicación de alto impacto.(Universidad Católica de Cuenca., 2026) Sánchez Guzmán, Ana Gabriela.Item type: Ítem , Access status: Embargo , Item type: Ítem , Access status: Acceso Abierto , Variación de los efectos de la melatonina sobre la motilidad y funcionalidad del semen ovino refrigerado(Universidad Católica de Cuenca., 2026) Asanza Dominguez , Karen PaolaMelatonin is a potent indolaminic antioxidant that protects cell membrane integrity against oxidative stress and stabilizes mitochondrial functionality. To determine the functional dose of melatonin on chilled ram semen based on the variation of its effect on motility and sperm functionality was the objective of this study, evaluating various concentrations on Katahdin and Blackbelly ram semen at 5 °C. A logarithmic scale from 1/16 mM to 16 mM was used, with 8 replicates during 2, 4, and 24 hours. Results demonstrated that melatonin acts in a dose-dependent manner under a second-grade polynomial model as a function of log₂, reaching its maximum biological efficacy at 4 hours. During this period, optimal values were recorded for vitality (80%) with 1.46 mM, progressivity (40.20%) with doses near 16 mM, and linearity (38.06%) with 0.5 mM. The parabolic behavior of linearity warns of a biphasic effect, where excessive concentrations are counterproductive for cellular kinematics. It is concluded that a concentration of 1.5 mM represents the optimal balance to preserve the structure and displacement capacity of the ovine sperm in these breeds, being the suggested dose for subsequent cryopreservation. Keywords: Melatonin, cryopreservation, linearity, vitality, progressivenessItem type: Ítem , Access status: Acceso Abierto , Desempeño productivo y reproductivo de la hibridación Dorper X Corriedale Mestiza, y Pelibuey X Corriedale Mestiza(Universidad Católica de Cuenca., 2026) Veintimilla León, Luis EstebanThe hybridization Dorper × Corriedale Mestiza and Pelibuey × Corriedale Mestiza was evaluated in the parish of Jerusalén, Biblián-Azogues, as part of a genetic improvement program for local Andean sheep lines. Progeny performance was analyzed through productive parameters such as birth weight and body condition, as well as reproductive parameters in the dams, including pregnancy rate and mortality rate. A total of 50 Corriedale Mestiza ewes were inseminated using a 14-day synchronization protocol and a laparoscopic insemination technique. Pregnancy diagnosis was performed by ultrasonography at 35 days post-insemination, and lambs were evaluated during the first 72 hours postpartum. The results demonstrated the viability of these hybridizations through artificial insemination, where the heritability coefficient of the dams was a determining factor in the reproductive characteristics of the offspring. Mortality indices highlighted the importance of prior deworming before synchronization and vitamin supplementation, which contributed to positive outcomes and reduced mortality. It was concluded that selecting dams with higher body weight and good body condition improves the success of genetic improvement programs, while artificial insemination reduces inbreeding and enhances productive and reproductive traits. These findings demonstrate the feasibility of genetic improvement programs and their contribution to strengthening Andean sheep production systems as a key component of sustainable livestock development. Keywords: Progeny, hybridization, heritability, artificial inseminationItem type: Ítem , Access status: Acceso Abierto , Evaluación de la curva de sobrevivencia espermática en un cooler en diferentes intervalos de tiempo(Universidad Católica de Cuenca., 2026) Suqui Pinos, Michael XavierSheep production in Ecuador is experiencing a sustained decline in the national flock, coupled with constraints limiting the implementation of reproductive biotechnologies. This study evaluated the sperm survival curve of ram semen diluted with Triladyl® and refrigerated at 5°C in a cooler under field conditions. Viability (Eosin–Nigrosin), membrane integrity (HOST), and progressive motility were assessed from 0 to 24 hours using ANOVA (p<0.05) and linear regression analyses. The research was conducted at Universidad Católica de Cuenca within a community outreach project aimed at reactivating sheep production through the establishment of a germplasm bank using ejaculates from fertility-proven rams collected via artificial vagina. No significant differences were detected among the evaluated parameters; however, regression analyses revealed a gradual decline beginning at 2–6 hours, followed by subsequent stabilization. It is concluded that cooler refrigeration preserves viable seminal parameters for up to 24 hours, representing an accessible alternative for reproductive programs under real production conditions in the Andean region. Keywords: Ecuador; Ejaculates; Ovine; RefrigerationItem type: Ítem , Access status: Acceso Abierto , Evaluación del Efecto Combinado de Menaquinona 4 y Coenzima Q10 sobre la Crioconservación de Semen Ovino(Universidad Católica de Cuenca., 2026) Castro Sánchez, Dayana PatriciaThis experimental study analyzed the impact of the combined use of two antioxidants, menaquinone-4 and coenzyme Q10, on the quality of ovine semen subjected to cryopreservation, comparing them with the combined effect of each molecule together with L- carnitine and their individual use. This model allowed the evaluation of possible redox effects of the combinations and their saturation in the medium due to their high molecular weight. The effects of these six combinations were assessed in eight ejaculates, analyzing sperm motility, membrane integrity, and cell viability through eosin-nigrosin staining, propidium iodide, rhodamine, and motility evaluation using the CASA system. The results demonstrated a negative effect of the molecular combinations on sperm quality, attributable to medium saturation and increased ionic load, which generated damage to the sperm membrane. It was concluded that limited use of antioxidants in semen extenders is recommended. Keywords: L-carnitine; molecular weight; combined effect; sperm membraneItem type: Ítem , Access status: Acceso Abierto , Producción de Larvas de Mosca (Musca domestica) como Suplemento en Aves de Postura de Traspatio(Universidad Católica de Cuenca., 2026) Padilla Duchi, Jessica ElizabethThe fastest-growing livestock sector in Ecuador is poultry production, driven by the high demand of its products and by-products. Feed management represents a critical component of this production system, (as it determines the extent to which birds can express their productive potential.) This research aimed to evaluate the effect of fly larvae (Musca domestica) supplementation on egg production and quality in backyard laying hens. The larvae were obtained through a controlled rearing system, yielding 1 kg per week, which were collected and dehydrated. One kilogram of this supplement was added weekly to the birds’ diet over a continuous six-week period, resulting in a significant increase (p<0,05) in weekly egg production. A total of 365 eggs were produced, compared to 259 eggs in the control group that did not receive the mentioned supplement, representing a 48,65% increase relative to T2. In addition, supplementation with larvae improved yolk pigmentation of the eggs, reaching values of 15 according to the YolkFan scale, compared to values of 10 in the control group. This change may be associated with increased dietary protein levels, which enhance carotenoid assimilation and improve yolk pigmentation in hen eggs. These findings support the hypothesis that insect-based supplements enhance poultry production. Keywords: Musca domestica, backyard poultry, poultry nutrition, YolkfanItem type: Ítem , Access status: Acceso Abierto , Dexametasona como antiemético en pacientes quirúrgicos caninos tratados con protocolos postoperatorios de morfina(Universidad Católica de Cuenca., 2026) Alvarez Peñaloza, Karla GermaniaPostoperative nausea and vomiting (PONV) represent a common complication in canines undergoing ovariohysterectomy (OVH), particularly when analgesic protocols involving morphine are utilized. This experimental study evaluated the efficacy of dexamethasone as an emetic prophylactic in 20 clinically healthy mixed-breed female dogs undergoing OVH under general anesthesia. The subjects were divided into four groups: two received preoperative administration of dexamethasone (0.2 mg/kg), while the remaining two received only morphine (0.2mg/kg) via subcutaneous injection and constant rate infusion (CRI). Clinical signs of PONV (emesis, nausea, and ptyalism), as well as their frequency and time of onset, were recorded. The results demonstrated a higher incidence of clinical signs in the groups receiving morphine alone—particularly via continuous infusion where the prophylactic administration of dexamethasone significantly reduced the frequency of PONV (p=0.02), showing a lower occurrence of nausea and emesis. These findings suggest that dexamethasone is an effective alternative for preventing opioid-induced PONV in canines, thereby contributing to enhanced postoperative recovery and animal welfare. Keywords: Dexamethasone; Morphine; Postoperative Nausea and Vomiting; Dogs; Continuous Infusion.Item type: Ítem , Access status: Acceso Abierto , Tipificación sanguínea en caninos (CANIS LUPUS FAMILIARIS) mestizos con el método rápido blood typing card en el refugio Arca-Cuenca(Universidad Católica de Cuenca., 2026) Calle Saeteros , Walter RománA study was conducted on blood typing in mixed-breed dogs at the Arca-Cuenca shelter, using the rapid method known as “Blood Typing Card” with the objective to determine the proportion of each blood type and establish its predominance in relation to sex and coat type. Blood samples were taken from the cephalic vein of 60 canines, of which 83% had Dea type 1.1 and 17% had Dea type 1.2, with no records of Dea type 1.3. The blood group distribution showed a slight majority of females in Dea type group 1.1 and a higher proportion of males in Dea type group 1.2. In addition, the Dea type group 1.1 predominated in short- haired canines. Significant correlations were found between sex and blood type, as well as correlations between blood type and coat type. These findings suggest a low genetic diversity in the analyzed population due to the predominance of the Dea 1.1 blood group (83%) and the absence of other blood types, suggesting a close genetic relationship and possible inbreeding in the analyzed population, possibly due to inbreeding, and highlighting the importance of blood typing in improve veterinary care in the shelter. The main conclusion was that blood typing in mixed-breed canines from the Arca-Cuenca shelter revealed that the most common blood group is Dea 1.1 (83%), with a slight majority of females in this group, meanwhile Dea 1.2, predominantly in males, showed a relationship with coat type, suggesting significant correlations between blood type, sex and phenotypic traits in the analyzed population. Keywords: typing, blood, ArcaItem type: Ítem , Access status: Embargo , Uso de la clipnosis para la inmovilización de gatos en la consulta clínica(Universidad Católica de Cuenca., 2026) Román Sánchez, Daniel Alejandro...Item type: Ítem , Access status: Acceso Abierto , Proyecto de Titulación embargado con fines de publicación de impacto.(Universidad Católica de Cuenca., 2026) Rubio Tapia , Daniela Michelle.Item type: Ítem , Access status: Acceso Abierto , Uso de cannabidiol como agente reductor de ansiedad en caninos con Hiperapego(Universidad Católica de Cuenca., 2025) D'Vries Toral, Erick JohanItem type: Ítem , Access status: Acceso Abierto , Evaluación del Efecto Combinado de la menaquinona-4 y L-carnitina sobre la Crioconservación del Semen Ovino(Universidad Católica de Cuenca., 2025) Lema Guamán, María FlorThis biological research was conducted at the Reproduction Laboratory of the Universidad Católica de Cuenca, Ecuador, and focused on the cryopreservation of ram genetics, a process that negatively affects sperm functionality and viability due primarily to oxidative stress (OS) generated during freezing and thawing, as a consequence of the biochemical properties of the sperm plasma membrane. The study evaluated the post-thaw effect of L-Carnitine (1 mM) and Menaquinone-4 (1 mM), individually and in combination (1 mM × 1 mM), on ovine semen collected from a single ram, assessing vitality, viability, plasma membrane integrity, mitochondrial activity, and sperm kinetics using the Computer Assisted Sperm Analysis (CASA) system. Significant differences (p<0.05) were detected in several parameters, where the membrane integrity (%HOST, p=0.002) indicated a negative effect associated with ionic imbalance, the high immotile index (p=0.047) reflected excess additives in the extender, and the reduced linearity (%LIN, p=0.005) was related to the absence of cofactors supporting antioxidant activity. The high variability among internal and external factors (replicates) affecting sperm performance suggests that antioxidant dosage and exposure times must be considered to better understand their synergistic or antagonistic biological effects under oxidative stress conditions. Keywords: Antioxidant; Ecuador; Biological Effects; Oxidative Stress; Genetics; Biological Research.
