Trabajos de Titulación - Medicina Veterinaria
URI permanente para esta colecciónhttps://dspace.ucacue.edu.ec/handle/ucacue/53
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Ítem Acceso Abierto Efecto biocida del extracto de raíz del barbasco (lonchocarpus utilis)en el control de piojos en cuyes(Universidad Católica de Cuenca., 2025) Calle Encalada, Juan David; Cuenca Condoy, Mercy; 0302606504The study evaluated the biocidal effect of Cubé resin (Lonchocarpus utilis) for controlling lice in guinea pigs. A total of 180 improved-genotype guinea pigs in the growth phase (400 ± 50 g) were used, distributed across six treatments: T0 (control), T1 (25%), T2 (20%), T3 (15%), T4 (10%), and T5 (5%), with three replicates of 10 guinea pigs each. Cubé resin underwent collection, cleaning, drying, crushing, pulverization, and sieving. Its quality and toxicity were assessed through a bioassay with Artemia salina, determining an LC50 of 0.50% at three hours. The biocidal efficacy was tested in two phases. In the in vitro phase, 150 lice were exposed to five concentrations (T1, 100%; T2, 80%; T3, 60%; T4, 40%; and T5, 20%), with 10 lice per concentration. In the in vivo phase, infested guinea pigs were treated with Cubé resin at 25%, 20%, 15%, 10%, and 5% at a dose of 1% of their live weight, and lice mortality was evaluated at 6, 12, and 18 hours. The in vitro results showed significant differences (p<0.05), achieving the highest mortality (100%) at the 100% concentration after 18 hours. In the in vivo phase, all concentrations reached 100% mortality at 6 hours, whereas the commercial product achieved its maximum mortality at 12 hours. It is concluded that Cubé resin at concentrations below 25% has a biocidal effect and could serve as an alternative to commercial treatments. Keywords Lonchocarpus utilis, Barbasco, Lice, Guinea pigs..Ítem Acceso Abierto Efecto de la concentración de la L-carnitina en congelación del semen ovino(Universidad Católica de Cuenca., 2025) Valdez Pinoargote, Girabel Gissela; Moscoso Piedra, Andrés Leonardo; 0804298040Sperm cryopreservation is a key technique in animal reproduction, particularly in ruminants, enabling assisted reproduction and benefiting veterinary medicine. L-Carnitine, an amino acid derived from lysine and methionine, plays a crucial role in preserving membrane integrity and mitochondrial function. The aim of this study was to assess the effect of Lcarnitine supplementation at doses of 1M, 0.750mM, 0.500mM, and 0.100mM on the sperm quality of three sheep breeds (Dorper, Kathadin, and Pelibuey). Semen was collected from each sheep over multiple mounts, resulting in three ejaculates per animal, with a total of nine samples, and a control test with semen free of L-carnitine. These samples were evaluated through laboratory tests (Eosin-Nigrosin, Propidium Iodide, and Rodamine) and analyzed using the CASA system to measure parameters such as PR, NP, IM, VCL, VAP, VSL, STR, LIN, ALH, and BCF. The results favored the 1M dose (highest concentration) across permeability, viability, and kinetics tests. Statistical differences were found in the LIN and STR values, consistently favoring the 1M concentration compared to the control. Keywords: Cryopreservation; L-Carnitine; Sheep; SpermatozoaÍtem Acceso Abierto Resistencia y Acritud de Pasteurella multocida en gatos domésticos (felis catus) en cuatro clínicas veterinarias del sur de Cuenca(Universidad Católica de Cuenca., 2025) Tinoco Suscal, Mateo Fabián; Aguilar Caivinagua, Santiago Andrés; 0105993687Pasteurella multocida is a zoonotic pathogen of high relevance in public health; however, information on its antimicrobial resistance (AMR) profiles and virulence factors in domestic cats is limited. This study aims to contribute to understanding these critical aspects, which are fundamental for developing effective strategies to prevent and control zoonoses. This study evaluates the AMR and virulence factors of P. multocida isolated from the oral mucosa of Felis catus in four veterinary clinics in southern Cuenca. Fifty oral mucosa samples were collected using sterile swabs, and susceptibility to 10 commonly used antimicrobials in veterinary medicine was analyzed. MALDI-TOF mass spectrometry was used to confirm the specific presence of Pasteurella multocida and bacterial load. The results will reveal the AMR profiles and virulence factors of the isolated strains, evaluating their potential zoonotic impact and implications for public health. This research will significantly contribute to the knowledge of the epidemiology of P. multocida in felines, identifying effective antimicrobials, those with resistance, and the present virulence factors. This research will significantly contribute to the knowledge of the epidemiology of Pasteurella multocida in Felis catus populations, identifying antimicrobials with therapeutic efficacy and those for which resistance is evident, as well as virulence factors. Keywords: Bacteria; Zoonosis; Antimicrobial Agents; Oral Mucosa; Virulence; Public Health; Mass Spectrometry; AMR; Felis Catus; Veterinary MedicineÍtem Embargo Evaluación de la resistencia antimicrobiana en cepas de staphylococcus aureus aisladas de la mucosa en un gato de las pampas (Leopardus garleppi)(Universidad Católica de Cuenca., 2025) Tinizhañay Chicaiza, Aracely Pilar; Maldonado Cornejo, Manuel Esteban; 0302934534.Ítem Embargo Evaluación de los parámetros productivos, control de temperatura y niveles de amoníaco en una producción avícola con cama nueva y cama reutilizada(Universidad Católica de Cuenca., 2025) Valarezo Ramirez, Hender Isaac; Cuenca Condoy, Mercy del Cisne; 0706104999.Ítem Embargo Respuesta superovulatoria de un protocolo tradicional con remplazo parcial de pluset (FSH-LH) por una dosis de eCG en bovinos(Universidad Católica de Cuenca., 2025) Suárez Ortiz, María Julissa; Alvarado Alvarado, Juan Carlos; 0350097101.Ítem Embargo Efecto biocida del Barbasco (Lonchocarpus Boophilus microplus) en el control de garrapatas (Riphicephalus Boophilus microplus)(Universidad Católica de Cuenca., 2025) Torres Lozano, Oscar Lennin; Cuenca Condoy, Mercy del Cisne; 1400715551Ítem Acceso Abierto Tiempo de infiltración de los huevos de toxocara canis en función del tipo de suelo(Universidad Católica de Cuenca., 2025) Villa Lucero, Marcia Catalina; Quinteros Rodas, Wilson Olmedo; 0104760509A study was carried out to determine the level of infiltration of Toxocara canis spp. eggs in three types of soil, as a contamination factor in soils or green areas, through an experiment that artificially simulated the parasite’s mobility in the laboratory. Eggs from the specimen were identified in fecal samples, and refined sawdust was used as a biosimulator material, which were evaluated together using the Faust Method. The three types of soil —sand, humus, and common soil (silt loam)— were placed in a cylindrical PVC tube measuring 12.7 cm (5 inches) x 20 cm in height, where the material was deposited. For 10 days, the samples were evaluated at three depths (5 cm, 10 cm, and 15 cm) to determine the degree of infiltration of Toxocara canis eggs. The results show positive cases for each type of soil, regardless of the soil type, with a higher number of positive cases at a depth of 5 cm. The presence and absence of simulant particles smaller than 100 um were evaluated, and finally, Toxocara eggs were identified at a depth of 5 cm. It is concluded that soils supersaturated with water inhibit infiltration, with higher infiltration rates observed after the first irrigation due to natural runoff. This suggests that the parasite could survive at these levels without exposure to sunlight, making this a preliminary study to understand the mobility of Toxocara canis in different soil types and thus preserve public health. Keywords Biosimulator, Faust Method, Humus, Public Health.Ítem Embargo Efecto de la menaquinona 4 y coenzima Q10 en la calidad espermática de semen porcino(Universidad Católica de Cuenca., 2025) Tapia Benavides , Freddy Josué; Alvarado Alvarado, Juan Carlos; 0107159311.Ítem Embargo Variación de PH y pérdidas por goteo en la premaduración cortes bovinos procedentes del centro de faenamiento del cantón Cañar(Universidad Católica de Cuenca., 2025) Suárez Vásquez, Susana Carolina; Maldonado Cornejo, Manuel Esteban; 0302856745.Ítem Acceso Abierto Estudio retrospectivo de pacientes caninos con pancreatitis aguda (cPL anormal)y su correlación con cambios en la biometría y química sanguínea(Universidad Católica de Cuenca., 2025) Bravo Chimbo, Jennifer Michelle; Aguilar Caivinagua, Jennifer Michelle; 0107432031Acute pancreatitis (AP) is a common disease in dogs, with variable severity and a significant mortality rate ranging from 27% to 58% of cases. Secondary complications, such as azotemia, are a major cause of morbidity and mortality in these patients. This study aimed to analyze the relationship between AP cases in dogs and changes in hematologic and blood chemistry parameters. A retrospective study was conducted at the Austrovet veterinary clinic, where data were collected from 33 medical records of canine patients diagnosed with AP using the canine pancreatic lipase (cPL) test between 2022 and 2023. The prevalence of azotemia and its correlation with hematologic and blood chemistry parameters were determined. The results of this study showed that red blood cell count, hematocrit, and hemoglobin levels were below normal values. Non-regenerative anemia was the most prevalent hematologic disorder, followed by neutrophilia and leukocytosis, suggesting an active systemic inflammatory response. Regarding blood chemistry, azotemia was frequently observed in 36.36% of cases, which could indicate renal impairment. Elevated alkaline phosphatase (39.39%) and serum amylase levels exceeding three times the normal range (39.39%) were other common findings, suggesting the presence of cholangitis and/or pancreatic damage. Thus, these results highlight the importance of hematology and blood chemistry in the early diagnosis of AP, as these abnormalities indicate severe complications such as azotemia. Timely diagnosis and appropriate treatment could help reduce mortality and improve the prognosis of dogs with acute pancreatitis. Keywords: Acute pancreatitis; Azotemia; Hematology; Blood chemistryÍtem Embargo Microdosis de coenzima Q10 como potencializador de la calidad espermática de semen ovino crioconservado(Universidad Católica de Cuenca., 2025) Quinche Guazhambo , Mónica Daniela; Moscoso Piedra, Andrés Leonardo; 0106979446.Ítem Embargo Efecto de hembras estrogenizadas sobre el comportamiento sexual y la calidad seminal del carnero.(Universidad Católica de Cuenca., 2025) Labanda Sigcho, Jorge Eduardo; Moscoso Piedra, Andrés Leonardo; 0107329237.Ítem Acceso Abierto Evaluación de tres dilutores comerciales sobre el semen de ovino post congelación(Universidad Católica de Cuenca., 2025) Paucar Quito, Janneth Elizabeth; Alvarado Alvarado, Juan Carlos; 0106540495Sheep breeding has a significant impact on the country's economy; however, traditional management practices are often maintained in this industry, which reduces international competitiveness. For this reason, it is essential to improve sheep production through the genetic improvement of animals with high productive and reproductive potential. The objective of this research was to evaluate the efficiency of three commercial dilutors, Ovixcell, Andromed and Triladyl on the sperm quality of post-freezing ovine semen. According to the parameters of each commercial house in semen from rams of the Katahdin, Pelibuey and Dorper breeds, with 15 extractions, 5 for each sheep, for a total of 45 samples, evaluated by traditional microscopy by staining, fluorescence, pH, also included kinetic parameters evaluated by the CASA system, all variables were evaluated at two intervals, in fresh semen freshly diluted and post-freezing. In the analysis of permeability and progressivity in fresh diluted semen, Triladyl obtained efficient results (p< 0.05), while in kinetics Andromed and Triladyl achieved a remarkable impact (p< 0.05), when analyzing the mentioned parameters in post-freezing Triladyl obtained significant results (p< 0.05). 05) while the other controls rotated, Ovixcell in second place demonstrating better resistance to cryopreservation (p< 0.05) before Andromed, which has evidenced a significantly drastic decrease, indicating high mortality in thawed semen. It is concluded that the Triladyl extender better preserves the functional characteristics of ovine sperm by protecting the plasma membrane, conserving vitality and providing greater protection against the consequences of cryopreservation. Keywords: sheep, sperm, diluent, cryopreservationÍtem Embargo Proyecto de titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Guiracocha Viñanzaca, Ingrid Michelle; Moscoso Piedra, Andrés Leonardo; 0107568669Ítem Acceso Abierto Efecto analgésico perioperatorio de la combinación de bupivacina al 0.5% y fentanilo por vía epidural lumbosacra en perras sometidas en ovario-histerectomía(Universidad Católica de Cuenca., 2025) Bastidas Cárdenas , Jorge Andrés; Castillo Hidalgo, Edy Paul; 0150533602Ovariohysterectomy (OVH) is a common surgical procedure in female canines, associated with intense pain due to the tissue trauma sustained during the operation. Consequently, it is essential to employ effective methodologies for preoperative analgesia. In these cases, epidural anesthesia has been recognized as a fundamental approach to alleviate postoperative discomfort due to its advantages. Therefore, the objective of this research was to evaluate the perioperative analgesic efficacy of a combination of 0.5% bupivacaine and fentanyl (50 mcg/ml), administered via the lumbosacral epidural technique, in canines undergoing ovariohysterectomy. For this purpose, apparently healthy patients aged between 1 and 5 years were selected and classified as ASA I according to the criteria established by the American Society of Anesthesiologists. The subjects were randomly distributed into two groups: the B+F group (n = 5) received a lumbosacral epidural administration of 0.5% bupivacaine combined with fentanyl (50 mcg/ml), and the B group (n = 5) was treated with 0.5% bupivacaine alone using the same route. The results showed statistically significant differences (p<0.05), where the B+F group treatment demonstrated a lower average score on the Glasgow pain scale (1.27 ± 0.87) compared to the B group (2.73 ± 0.87). These findings suggest that the combination of bupivacaine and fentanyl not only improves pain control but also may reduce the need for additional doses of anesthetics and analgesics (such as NSAIDs) during the postoperative period. Keywords: Pain relief; Canine; Ovariohysterectomy; Glasgow Pain Scale.Ítem Acceso Abierto Lactato en sangre periférica en pacientes críticos, componente esencial del protocolo de estabilización guiada a metas(Universidad Católica de Cuenca., 2025) Rodriguez Muñoz, Mónica Nicole; Rodríguez Muñoz , Mónica Nicole; 0350149498; Castillo Hidalgo EdyThe management of critically ill patients in veterinary medicine requires rapid identification of complications for effective decision-making that benefits their health. Blood lactate is a key biomarker in evaluating hypoperfusion, metabolic acidosis, and organ damage, with well-established applications in human medicine, but still limited in veterinary care. This study evaluated peripheral blood lactate levels as a prognostic indicator in 30 critically ill canine patients with multiple causes, admitted to the "Le Peluts" Veterinary Specialty Clinic in Azogues. Lactate levels were measured upon admission and at 12 hours after treatment as part of a goal-directed stabilization protocol. A significant reduction (p≤0.05) in lactate levels was observed, from 4.34 mmol/l (+3.85) to 2.14 mmol/l (+2.96), indicating a positive therapeutic response and clinical improvement. A strong correlation was also found between lactate, heart rate, and prognosis score for each patient, with higher lactate levels linked to unfavorable outcomes. Additionally, mucosal coloration was associated with better prognoses, reinforcing lactate’s role as a dynamic tool in clinical decision-making. This emphasizes the importance of integrating lactate monitoring into stabilization protocols. This approach not only improves survival rates but also optimizes management and decisionmaking in critically ill veterinary patients. Future research could explore differentiating lactate levels based on pathology type, enhancing its potential as a prognostic indicator for various conditions, ultimately improving clinical outcomes and treatment strategies. Keywords: Biomarker; Hypoperfusion; PrognosisÍtem Acceso Abierto Concentración de cortisol como bioindicador de bienestar animal en la manada equina expuesta a estímulos sociales(Universidad Católica de Cuenca., 2025) Padrón Pesantez, María Eulalia; Maldonado Cornejo, Manuel Esteban; 0150175420Equines are highly sensitive to stress, meaning that any activity around them can trigger a release of plasma cortisol, thus impacting their well-being. Research on this subject has primarily focused on the utilitarian aspect of these animals, which often involves exploiting them beyond their natural capacities and consequently compromising their health. This study aimed to determine serum cortisol levels using the quantitative ELISA (Accubind) technique in a herd of horses used for equine-assisted therapy, compared to a stable group at the same location, measured at different times of the day as a bioindicator of animal welfare. For this purpose, eight castrated male horses from the same herd were selected, with four participating in equine therapy (experimental group) and four not (control group). Blood samples were drawn twice daily over five different days, resulting in a total of 80 samples. The cortisol range in the experimental group was 10.97 ± 5.73 μg/dL, while in the control group, it was 9.03 ± 3.99 μg/dL (p≥0.05). A significant difference was found between groups when comparing the interaction of circadian rhythm (morning and afternoon) (p<0.05). Horses in the control (10.21 μg/dL) and therapy groups (11.94 μg/dL) began with similar values; however, the control group showed a decrease (7.84 μg/dL) in cortisol levels by the end of the day, unlike the therapy group (9.99 μg/dL). These results indicate that equine therapy affects cortisol levels, possibly due to the physical effort involved, which may disrupt the animals' circadian rhythm. Keywords: ELISA; Stabled; Equine-Assisted Therapy; Circadian RhythmÍtem Acceso Abierto Efecto de la fitoestrógenos de la alfalfa en la espermatogénesis de los cobayos machos(Universidad Católica de Cuenca., 2024) Cajamarca Ortega, Josue Ismael; Bravo Alvarez , María Ximena; 0302568365In this research, alfalfa phytoestrogens in the pre-flowering stage are examined because they affect spermatogenesis and the hormonal profiles of male guinea pigs. It aims to evaluate phytoestrogen through diet. Three experimental groups were created. The first group was fed with alfalfa in pre-flowering, the second group was fed with free balanced alfalfa, and the third was fed with a combination of the diets. In consequence, the levels of testosterone and estradiol were measured. Therefore, the diameter of the seminiferous tubules, Leydig cells, spermatogonia germ cells, spermatocytes, and spermatids for the guinea pigs that received alfalfa experienced a decrease in germ cell production and significant hormonal variability, especially in estradiol levels. The findings suggest that phytoestrogens can negatively affect the level of testosterone over a certain period. However, no significant differences were found in testosterone levels, with phytoestrogens being part of a balanced diet of essential proteins that provide ideal nutrients for reproduction, growth, and tissue development. In the same way as the plant diet, phytochemicals prevent damage to cells from diseases to systems such as the cardiovascular or nervous system; therefore, a balanced diet of nutrients is ideal. In this sense, food improvement is important for the breeding of guinea pigs by determining the source of phytoestrogens, affecting on the physiognomy, and achieving a positive effect on nutrition and reproduction.Keywords: Phytoestrogens; Alfalfa in pre-flowering; Hormonal profile; Testosterone; Estradiol. Keywords: Phytoestrogens; Alfalfa in pre-flowering; Hormonal profile; Testosterone; Estradiol.Ítem Acceso Abierto Comparación de dos sistemas de producción intensivo de pollos Broilers en ambiente controlado en Naranjito y Cruz de Hueso, Ecuador(Universidad Católica de Cuenca., 2024) Cargua Manguia, Alejandro Moises; Cargua Manguia, Alejandro Moises; 0940157522In this research, it was possible to analyze and determine the impact of altitude on the performance of broiler chickens in a controlled environment. A thorough comparison was made in two farms located at different altitudes, the first at 100 meters above sea level (Naranjito); the second was at 500 meters above sea level (Cruz de Hueso). Evaluation parameters such as the effects of temperature, humidity, wind speed, and gas levels (CO2 and NH3) on birth growth and health were established. The data provided in the findings indicate that, although the values are insignificant, the external environmental conditions had a minimal, almost imperceptible, influence of mortality and feed conversion ratio. In particular, lower external temperatures were observed in Avicelenes 2, negatively affecting the birds' energy expenditure and growth rates. According to the mentioned results, it is necessary to highlight the significance of considering altitude to optimize broiler production. Likewise, it is advisable to suggest specific management strategies for farms at different altitudes. Furthermore, it is highly recommended that subsequent studies analyze in detail the cost-benefit of broiler production at different altitudes, as well as the types of management that typically include diet, water, biosecurity, genetics, and infrastructure, all with the sole objective purpose of determining whether or not there are significant differences in prolonged research periods. Keywords Broilers, Houses, Altitude, Microclimate
