Trabajos de Titulación - Bioquímica y Farmacia
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- ÍtemAcceso AbiertoDeterminación de Staphylococcus aureus en bases líquidas de maquillaje expendidas en el mercado 9 de octubre de la ciudad de Cuenca- Ecuador(Universidad Católica de Cuenca., 2024) Andrade Arias , Mauricio Lenin; Cox Eras, María Sol; Araujo Campoverde, María Viviana; 0105689061; 2200237622Introduction: The microbiological safety of cosmetic products is a growing concern due to their direct contact with the skin and the possibility of causing infections if contaminated. In particular, Staphylococcus aureus (S. aureus) is a microorganism that, due to its ability to colonize the skin and mucous membranes, represents a significant health risk, especially in daily-use products such as liquid foundation. Objective: This research aimed to determine the presence or absence of S. aureus in liquid foundation sold in the “9 de Octubre” Market, Cuenca, Ecuador. Methodology: The research methodology was descriptive and observational, with a qualitative cross-sectional approach. Cultures were prepared using Petri dishes with Modified Letheen Agar (MLA) and seeded in duplicate. The colonies identified were transferred to mannitol salt differential agar and confirmatory biochemical tests were performed. Results: After analyzing 40 liquid foundation samples on differentiation agar (Mannitol salt), the presence of S. aureus was determined in only two different brands of the samples. Biochemical tests confirmed the presence of S. aureus in these samples. Conclusion: The analysis of the liquid foundation in this study determined a low prevalence of S. aureus. In conclusion, the positive results obtained are not representative to generalize the contamination of this cosmetic product
- ÍtemEmbargoProyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2024) Godoy Illescas, Angel Josue; Arteaga Sarmiento, Sandra Denisse; 0106315765
- ÍtemAcceso AbiertoEvaluación in silico de los derivados de flavonoides como inhibidores de la PBP2a Staphylococcus aureus resistente a la meticilina(Universidad Católica de Cuenca., 2024) Espinoza Reyes , Einsteen Angel; Carpio Arévalo, Juan Marcelo; 0705880466Introduction: Antibiotic resistance in methicillin-resistant Staphylococcus aureus generates infections that make treatment difficult, especially in patients with compromised immune systems. Therefore, molecular docking studies have been conducted as a new alternative to treat these infections and intervene in the virulence factors. Objective: To evaluate the inhibitory activity of flavones by an in silico essay against the Penicillin-Binding Protein 2a (PBP2a) enzyme of methicillin-resistant Staphylococcus aureus. Methodology: To develop this thesis, an in silico experimental design with a quantitative approach was used to evaluate the following aspects: Absorption, Distribution, Metabolism, Excretion (ADME) properties, interactions, and energy levels of the 30 flavones, using molecular docking to interact with PBP2a of methicillin-resistant Staphylococcus aureus. Results: The affinity of each flavone to interact with the PBP2a enzyme was evaluated by molecular docking, obtaining different energy levels of the 5 best molecules, ranging between 10.7 and 9 kcal/mol. Conclusion: The data collected in this study shows the antibacterial effects of flavones and their potential use as a new antimicrobial to aid in the treatment of methicillin- resistant Staphylococcus aureus infections.
- ÍtemEmbargoProyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2024) Gualpa Cox, Marcos Moises; Lopez Salazar , Juan Fernando; Arteaga Sarmiento, Sandra Denisse; 0942190398; 0105539621
- ÍtemAcceso AbiertoEvaluación del potencial cardiotóxico de los fitoquímicos de la planta Urginea marítima mediante los métodos computacionales.(Universidad Católica de Cuenca., 2024) Idrovo Hidalgo, Fausto Daniel; Lucero Arias , Pamela Katherine; Carpio Arévalo, Juan Marcelo; 0107455321; 0106983257Introduction: This research evaluated the cardiotoxic activity of phytochemicals from Urginea maritima, which is traditionally used in herbal medicine. However, the therapeutic application of these compounds requires a rigorous evaluation of their potential side effects. In the cardiovascular system, the crucial aspect to consider is the interaction of these phytochemicals with the hERG channel of the heart. Objective: To evaluate the cardiotoxicity of phytochemicas from U. maritima in humans, using Quantitative Structure Activity Relationship (QSAR) tools. Methodology: A quantitative and descriptive experimental research design was used to develop this research. A methodological approach including virtual tools such as QSAR, Pred-hERG, and ADMETlab 2.0, was used to predict the cardiotoxicity of the identified compounds. Results: It is suggested that certain phytochemicals from U. maritima, in addition to those already known, may have cardiotoxicity in humans. It was found that 23 phytochemicals act as blockers of the hERG channel in the myocardium. Among these, the top 10 with the highest reliability index were selected, with five molecules grouped in the bufadienolides group, one belonging to the glycosides, and another to the diterpenoids; for the remaining three, no literature was found that classified them. Conclusion: The need to continue contributing to the published scientific literature on these new advancements and consolidation approaches was corroborated, due to the limited information available on several of the compounds present in these medicinal plants, for which there is still no evidence on their cardiotoxicity.
