Trabajos de Titulación - Bioquímica y Farmacia
URI permanente para esta colecciónhttps://dspace.ucacue.edu.ec/handle/ucacue/27
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Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Rodas Bueno, Josseline Natalia; Zúñiga Tacuri, Mateo Andrés; Martínez León, Maritza del Rosario; 0106274954; 0106004625Ítem Acceso Abierto Evaluación de la susceptibilidad antifúngica y el efecto inhibitorio del extracto de propoleo frente a Aspergillus fumigatus(Universidad Católica de Cuenca., 2025) Guzmán Aldas, Joseline Carolina; Villa Gañay, Paola Alejandra; Bravo Crespo, David Israel; 0107376113; 0106480643Introduction: Propolis, known for its antifungal properties, has demonstrated efficacy against Aspergillus fumigatus, arousing the interest of modern medicine. Studies conducted in Peru and Ecuador highlight its antimicrobial and antioxidant potential. This study, conducted in Cuenca, aims to provide information on its effectiveness against A. fumigatus, contributing to scientific knowledge and potential local applications, positioning it as a promising resource in the medical and agro-industrial fields. Objective: To evaluate the inhibitory effect of propolis extract against A. fumigatus and determine its antifungal sensitivity to develop new pharmaceutical alternatives. Methodology: This study was conducted using a quantitative research approach with an experimental design. In vitro cultures were used to evaluate the inhibitory effect of propolis on Aspergillus fumigatus ATCC 20430 under controlled conditions. Observations were recorded at 24, 48, and 144 hours, testing various concentrations and solvents, and comparing results with conventional treatments and negative controls. The experimental design ensured uniformity and replicability. Results: The test revealed that propolis dissolved in glycerin significantly inhibited the growth of A. fumigatus. At concentrations of 30%, it achieved total inhibition within 24 hours and maintained a reduction of over 70% up to 144 hours. In comparison, alcohol and acetone showed lower persistence, while itraconazole and dimethyl sulfoxide (DMSO) showed more limited effects. Conclusion: Propolis extract demonstrated an inhibitory effect against Aspergillus fumigatus, highlighting its potential as an antifungal alternative. These findings support its potential use in the development of new pharmaceutical options.Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Perez Zambonino, Melissa Natalia; Rodas Chillogalli, Lizbeth Nayeli; Torres Segarra, Silvia Monserrath; 2350067480; 0105733018Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Cando Duchitanga, Sthefany Alexandra; Daza Vélez, Lizbeth Ivonne; Torres Segarra, Silvia Monserrath; 0107331407; 0302605258Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Palacios Bravo , María Paula; Zhunio Lema, Adrian Alejandro; Torres Segarra, Silvia Monserrath; 0151054228; 0350074381Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Guamán Pucha, Cristina Michelle; Rivera Tuba, Jonathan Xavier; 0105744999Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Tandazo Peralta, Gabriela Elizabeth; Bravo Crespo , David Israel; 0105503296Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Benavidez Llanos, Nayely Daniela; Jara Toledo, Diana Micaela; Torres Segarra, Silvia Monserrath; 0106758840; 0106573512Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Suconota Mora, Eva Fernanda; Espinoza Yupa, Gladys Valeria; Carpio Arévalo, Juan Marcelo; 0106835507; 0302487558Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Sangurima Paredes , Gloria Estefanía; Muñoz Sanmartin, Owen Rodrigo; Bravo Crespo, David Israel; 0106032972; 0706061108Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Realpe Yar, Jorge Arturo; Carpio Arévalo, Juan Marcelo; 1725155863Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2024) Heras Maldonado, Noelia Brishith; Illisaca Ramírez, Cinthya Belén; Vélez Zamora, Luis Alfredo; 0106156334; 0106505407Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2024) Peñaloza Fajardo, Walter Fabricio; Bermeo Coronel, Elizabeth Paola; Macías Matamoros, Andrea Fernanda; 0151045796; 0106694185Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2024) Huanca Cueva, Hillary Dayana; Ortiz Tejedor, Jonnathan Gerardo; 0706497807Ítem Acceso Abierto Análisis del sistema de gestión documental de calidad de laboratorios clínicos privados de la ciudad de Cuenca(Universidad Católica de Cuenca., 2024) Medina Santillán, Lizbeth Andrea; Leyton Delgado , Geanella Raquel; Martínez León, Maritza del Rosario; 0604070284; 0943665307Introduction: Document management is a crucial aspect of the functioning of clinical laboratories, as it ensures the quality and compliance of clinical results following national and international regulations. Objective: To analyze the compliance of quality indicators in the document management systems of private clinical laboratories in Cuenca following ISO 15189 standards and the guidelines of the Ministry of Public Health (MSP, by its Spanish acronym). Methodology: The applied study was qualitative and cross-sectional, including surveys in low-complexity laboratories to evaluate document quality indicators. Results: The clinical laboratories demonstrate high levels of compliance in permission control (91.7%) and quality records (100%) according to regulations but moderate compliance in non-conformity management and corrective actions (58%). The main limitations include insufficient resources and training, inadequate infrastructure, and low adherence to non-priority indicators. Conclusion: The document management indicators of clinical laboratories were analyzed, showing notable compliance with several parameters of ISO 15189 standards and MSP guidelines. Although quality and technical records achieved 100% adherence, areas for improvement were identified in detecting non- conformities and in corrective and preventive actions. The main limitations include insufficient resources and training, inadequate infrastructure, and low adherence to non-priority indicators.Ítem Acceso Abierto Ambientes domésticos de la Costa Ecuatoriana como reservorios de levaduras potencialmente patógenas(Universidad Católica de Cuenca., 2024) Suña Solano, Evelyn Johanna; Vega Lema, Bryan Fabricio; Buela Salazar, Lenys Margarita; 0350274965; 0302323936Introduction: Infectious diseases are one of the leading causes of global mortality, transmitted by microorganisms in human environments. Urbanization has altered human exposure to these microorganisms, affecting immune health. Domestic environments, such as dishwashers and drains, serve as reservoirs of extremophile microorganisms capable of surviving in extreme conditions. Objective: To determine the diversity of cultivable yeasts that colonize extreme domestic environments in the coastal region of Ecuador. Methodology: Samples were collected from biofilms, drains, and dish dryers, followed by primary isolation, which was cultured on Sabouraud Dextrose agar. Each isolate was characterized morphologically, physiologically, and biochemically. Before characterization, virulence factors were evaluated, and molecular techniques were used to identify the species to which it belongs. Results: Ten locations were selected, from which 28 microbial strain isolates were obtained. From these strains, 12 yeasts were identified, and various virulence factors were determined, including hemolysin, protease, and lipase. PCR amplification of the ITS region revealed that the most common strains belonged to the genera Candida metapsilosis and Kodamaea ohmeri. Conclusion: The prevalence of pathogenic yeasts in domestic environments is highlighted, which has implications for human health in the coastal region of Ecuador.Ítem Acceso Abierto Análisis del sistema de gestión de calidad técnica en fase preanalitica, en laboratorios clínicos privados de la ciudad de Cuenca.(Universidad Católica de Cuenca., 2024) Figueroa Balladares, Diana Fernanda; Rodríguez Barrera, Jesenea Paulina; Guamán Sánchez, María Fernanda; 0150062479; 0605221308Introduction: It is important to implement a technical quality management system in the pre-analytical phase of private clinical laboratories to reduce and avoid errors and guarantee reliable and efficient results in laboratory tests. Objective: This research aimed to analyze the technical quality management systems of private clinical laboratories in the city of Cuenca. Methodology: To develop this thesis, a qualitative, observational, descriptive- explanatory research design is used, conducted through quality management audits of private clinical laboratories in Cuenca that have an operating permit granted by the ACESS (Agency for Quality Assurance in Health Services and Prepaid Medicine). Results: It was observed that 70% of the intervened laboratories comply with the technical quality standards, although the majority of laboratories comply with the standardized request and sample handling, only 80% correctly follow the identification procedures, and none keeps a daily record of sample entry. Conclusion: The analysis of the technical quality management systems in the private clinical laboratories of Cuenca shows general compliance with the ISO 15189:2012 Standard, although areas for improvement persist. The least met indicators are related to traceability and internal control. In addition, limitations were identified such as lack of resources, insufficient training and difficulties in updating procedures. It is essential to implement continuous improvement strategies to optimize technical and operational quality in the city's laboratories.Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2024) Jimbo Condo , Mateo Josué; Moran Prado , Michael Esteban; Torres Segarra, Silvia Monserrath; 0106110539; 0105308381Ítem Acceso Abierto Presencia de levaduras potencialmente patógenas en ambientes extremos domésticos del Oriente ecuatoriano(Universidad Católica de Cuenca., 2024) García Marín, Andrés Nicolás; Lazo Alvarado, Julio Humberto; Yarzabal Rodríguez, Luis Andrés; 0106434590; 0106047491Introduction: This research identified and characterized potentially pathogenic yeasts in extreme domestic environments, specifically in dishwashers in the Amazon region of eastern Ecuador. Objective: To evaluate the survivability of yeasts under adverse conditions and their potential to cause infections in humans Methodology: Dishwasher samples were collected from households and cultured on Sabouraud Agar to isolate yeasts. Subsequently, the isolates were morphologically and microscopically characterized, and virulence tests, such as hemolysis and phospholipase production, were performed. Molecular identification was conducted by amplification of the ITS region of the DNA, followed by sequencing and analysis with the BLASTN program to determine the species present. Results: The results showed the presence of several yeast species, including Candida and Rhodotorula. Some strains showed resistance to high temperatures (up to 40°C) and the ability to produce phospholipases and hemolysins. However, no proteolytic activity was detected in the isolates. Conclusion: The findings suggest that yeasts present in extreme domestic environments could potentially risk human health, especially in immunocompromised individuals.Ítem Acceso Abierto Análisis del sistema de gestión de calidad de laboratorios clínicos privados de la ciudad de Cuenca(Universidad Católica de Cuenca., 2024) Barrera Coronel , Stephanie Jhomayra; Mora Plaza , Gabriela Kimberly; Martínez León , Maritza del Rosario; 0107208506; 0106779366Introduction. Quality management in clinical laboratories is essential to ensure the reliability, safety, and validity of the results. This system includes a documental and technical component and is divided into three phases: pre-analytical, analytical, and post-analytical. The first phase is the most prone to errors. Objective. This research aimed to evaluate the compliance of private laboratories in Cuenca with the Quality Management System, focusing on the pre-analytical phase in accordance with ISO 15189 standards. Methodology. Non-probabilistic sampling was conducted, selecting 14 laboratories in the city to be audited. Descriptive statistics were applied for frequency analysis to obtain qualitative data. Results. Seventy-three percent (73%) of the laboratories complied with the parameters of the pre-analytical phase, while Document Management achieved 100% compliance, and Technical Management showed a compliance rate of 64%. The primary limitations found in these laboratories were a lack of personnel (28%), knowledge (44%), and resources (28%). Conclusion. The pre-analytical phase was the most problematic in terms of inaccuracies among the phases of analysis in Cuenca’s laboratories. The most significant challenges were the lack of personnel, scarcity of resources, and considerable lack of knowledge. Improvement in these areas could reduce errors and increase the accuracy of results in the city's clinical laboratories.
