Trabajos de Titulación - Bioquímica y Farmacia
URI permanente para esta colecciónhttps://dspace.ucacue.edu.ec/handle/ucacue/27
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Ítem Acceso Abierto Extremófilos en ambientes antropizados: Aislamiento de bacterias halotolerantes a partir de sal doméstica(Universidad Católica de Cuenca., 2025) Gómez Minchalo, Andrea Silvana; Morales Ulloa, Nicole Michelle; Yarzábal Rodríguez, Luis Andrés; 0150625424; 0605446863Introduction: Saline environments are home to extremophilic microorganisms, especially halophilic and halotolerant bacteria with biotechnological potential, thanks to their enzymes and metabolites. To isolate them, products from these environments can be used, such as unprocessed table salt. Objective: To detect the presence of viable and culturable microorganisms in table salt samples available in anthropized spaces (supermarkets). Methodology: Bacteria were isolated from table salt samples using Sabouraud Dextrose Agar and Nutrient Agar supplemented with NaCl. The isolates were morphologically characterized, and their halotolerance was evaluated. Molecular identification was performed by sequencing the 16S rRNA gene and conducting subsequent bioinformatic analysis. Results: Halotolerant microorganisms were detected in samples of Flor de Sal and smoked salt, with greater diversity observed in the former (13 morphotypes) compared to the latter (4 morphotypes). Seventeen isolates were purified, with 11 selected for further studies. The majority were Gram-negative bacilli, and yeasts and cocci were also detected. All isolates exhibited tolerance to NaCl concentrations ranging from 0.5% to 10.5% (w/v), but none grew at 20.5%. Bioinformatic analysis identified strains related to the species Priestia flexa, Bacillus boroniphilus, Bacillus aquimaris, Cytobacillus pseudoceanisediminis, Mesobacillus maritimus, Aureimonas altamirensis, Pantoea eucrina, and Kocuria rhizophila. Conclusion: Eight halotolerant isolates were identified, showcasing their ability to thrive at varying NaCl concentrations. Some strains exhibited biotechnological potential, such as the production of substantial exopolysaccharides. These findings emphasize the presence of significant microbial diversity in table salt and its relevance for biotechnological applications.Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Cantos Vásquez, Luis Eduardo; Heras Languidey, Xavier; Vélez Zamora, Luis Alfredo; 0350087136; 0302701693Ítem Acceso Abierto Caracterización fenotípica y ubicación filogenética de cepas de levaduras del género Rhodotorula y afines en ambientes domésticos de las diferentes regiones del Ecuador(Universidad Católica de Cuenca., 2025) Segarra Espinoza , Juan Carlos; Vásquez Díaz, Mario Esteban; Yarzábal Rodríguez, Luis Andrés; 0150565190; 0106990757Introduction: The growing interest in environmental microbiology has highlighted the importance of studying the complex diversity of microorganisms inhabiting domestic spaces. These environments, often overlooked in microbiological studies, harbor microbial communities whose presence can have significant and direct implications for the health of the inhabitants. Objective: To characterize yeast strains at the phenotypic and molecular levels belonging to the genus Rhodotorula and related species that colonize extreme domestic environments in different regions of Ecuador. Methodology: This study was conducted using an experimental and descriptive research design. Quantitative and qualitative approaches were used to phenotypically and genetically characterize the yeast strains of the genus Rhodotorula. Results: Primary isolation of different strains of the genus Rhodotorula was performed from domestic environments, specifically from kitchen sinks in several households in different regions of Ecuador. Conclusion: The data obtained in the present study support that kitchen sinks in domestic environments can be colonized by different genus Rhodotorula and Cytobasidium species, and these strains show affinity for certain virulence factors.Ítem Acceso Abierto Diseño, evaluación y optimización de estuches didácticos para el fortalecimiento de la enseñanza de microbiología y biotecnología(Universidad Católica de Cuenca., 2025) Vásquez Espinoza, Pablo Andrés; Méndez Andrade, Israel Sebastián; Yarzábal Rodríguez, Luis Andrés; 1401041445; 0107432577ntroduction: Scientific and technological development requires highly skilled professionals. However, conventional theoretical classes present limitations in the teaching of natural sciences, leading to disinterest and learning difficulties in students. In this study, didactic kits were designed and evaluated for academic performance and the understanding of fundamental concepts through scientific practice. Objective: To design, optimize, and evaluate the pedagogical capacity of didactic kits. Methodology: A literature search was conducted using Boolean operators in databases. Subsequently, experimental protocols were designed for the implementation of the kits. Their pedagogical effectiveness was evaluated through a pilot test with first-year Biochemistry and Pharmacy students at the Catholic University of Cuenca. Results: The post-test results showed a better performance in the experimental group, indicating statistical significance for most parameters. Therefore, the students in the experimental group improved after using the didactic kits. Conclusion: The didactic kits strengthen academic learning in the early years of the Biochemistry and Pharmacy program. Furthermore, the use of these kits stimulates the acquisition of novel theoretical knowledge, and contributes to a pedagogical and academic improvement Keywords: Didactic kits, methodology, teaching, scienceÍtem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Rodas Bueno, Josseline Natalia; Zúñiga Tacuri, Mateo Andrés; Martínez León, Maritza del Rosario; 0106274954; 0106004625Ítem Acceso Abierto Evaluación de la susceptibilidad antifúngica y el efecto inhibitorio del extracto de propóleo frente a Aspergillus fumigatus(Universidad Católica de Cuenca., 2025) Guzmán Aldas, Joseline Carolina; Villa Gañay, Paola Alejandra; Bravo Crespo, David Israel; 0107376113; 0106480643Introduction: Propolis, known for its antifungal properties, has demonstrated efficacy against Aspergillus fumigatus, arousing the interest of modern medicine. Studies conducted in Peru and Ecuador highlight its antimicrobial and antioxidant potential. This study, conducted in Cuenca, aims to provide information on its effectiveness against A. fumigatus, contributing to scientific knowledge and potential local applications, positioning it as a promising resource in the medical and agro-industrial fields. Objective: To evaluate the inhibitory effect of propolis extract against A. fumigatus and determine its antifungal sensitivity to develop new pharmaceutical alternatives. Methodology: This study was conducted using a quantitative research approach with an experimental design. In vitro cultures were used to evaluate the inhibitory effect of propolis on Aspergillus fumigatus ATCC 20430 under controlled conditions. Observations were recorded at 24, 48, and 144 hours, testing various concentrations and solvents, and comparing results with conventional treatments and negative controls. The experimental design ensured uniformity and replicability. Results: The test revealed that propolis dissolved in glycerin significantly inhibited the growth of A. fumigatus. At concentrations of 30%, it achieved total inhibition within 24 hours and maintained a reduction of over 70% up to 144 hours. In comparison, alcohol and acetone showed lower persistence, while itraconazole and dimethyl sulfoxide (DMSO) showed more limited effects. Conclusion: Propolis extract demonstrated an inhibitory effect against Aspergillus fumigatus, highlighting its potential as an antifungal alternative. These findings support its potential use in the development of new pharmaceutical options.Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Perez Zambonino, Melissa Natalia; Rodas Chillogalli, Lizbeth Nayeli; Torres Segarra, Silvia Monserrath; 2350067480; 0105733018Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Cando Duchitanga, Sthefany Alexandra; Daza Vélez, Lizbeth Ivonne; Torres Segarra, Silvia Monserrath; 0107331407; 0302605258Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Palacios Bravo , María Paula; Zhunio Lema, Adrian Alejandro; Torres Segarra, Silvia Monserrath; 0151054228; 0350074381Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Guamán Pucha, Cristina Michelle; Rivera Tuba, Jonathan Xavier; 0105744999Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Tandazo Peralta, Gabriela Elizabeth; Bravo Crespo , David Israel; 0105503296Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Benavidez Llanos, Nayely Daniela; Jara Toledo, Diana Micaela; Torres Segarra, Silvia Monserrath; 0106758840; 0106573512Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Suconota Mora, Eva Fernanda; Espinoza Yupa, Gladys Valeria; Carpio Arévalo, Juan Marcelo; 0106835507; 0302487558Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Sangurima Paredes , Gloria Estefanía; Muñoz Sanmartin, Owen Rodrigo; Bravo Crespo, David Israel; 0106032972; 0706061108Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2025) Realpe Yar, Jorge Arturo; Carpio Arévalo, Juan Marcelo; 1725155863Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2024) Heras Maldonado, Noelia Brishith; Illisaca Ramírez, Cinthya Belén; Vélez Zamora, Luis Alfredo; 0106156334; 0106505407Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2024) Peñaloza Fajardo, Walter Fabricio; Bermeo Coronel, Elizabeth Paola; Macías Matamoros, Andrea Fernanda; 0151045796; 0106694185Ítem Embargo Proyecto de Titulación embargado con fines de publicación de impacto(Universidad Católica de Cuenca., 2024) Huanca Cueva, Hillary Dayana; Ortiz Tejedor, Jonnathan Gerardo; 0706497807Ítem Acceso Abierto Análisis del sistema de gestión documental de calidad de laboratorios clínicos privados de la ciudad de Cuenca(Universidad Católica de Cuenca., 2024) Medina Santillán, Lizbeth Andrea; Leyton Delgado , Geanella Raquel; Martínez León, Maritza del Rosario; 0604070284; 0943665307Introduction: Document management is a crucial aspect of the functioning of clinical laboratories, as it ensures the quality and compliance of clinical results following national and international regulations. Objective: To analyze the compliance of quality indicators in the document management systems of private clinical laboratories in Cuenca following ISO 15189 standards and the guidelines of the Ministry of Public Health (MSP, by its Spanish acronym). Methodology: The applied study was qualitative and cross-sectional, including surveys in low-complexity laboratories to evaluate document quality indicators. Results: The clinical laboratories demonstrate high levels of compliance in permission control (91.7%) and quality records (100%) according to regulations but moderate compliance in non-conformity management and corrective actions (58%). The main limitations include insufficient resources and training, inadequate infrastructure, and low adherence to non-priority indicators. Conclusion: The document management indicators of clinical laboratories were analyzed, showing notable compliance with several parameters of ISO 15189 standards and MSP guidelines. Although quality and technical records achieved 100% adherence, areas for improvement were identified in detecting non- conformities and in corrective and preventive actions. The main limitations include insufficient resources and training, inadequate infrastructure, and low adherence to non-priority indicators.Ítem Acceso Abierto Ambientes domésticos de la Costa Ecuatoriana como reservorios de levaduras potencialmente patógenas(Universidad Católica de Cuenca., 2024) Suña Solano, Evelyn Johanna; Vega Lema, Bryan Fabricio; Buela Salazar, Lenys Margarita; 0350274965; 0302323936Introduction: Infectious diseases are one of the leading causes of global mortality, transmitted by microorganisms in human environments. Urbanization has altered human exposure to these microorganisms, affecting immune health. Domestic environments, such as dishwashers and drains, serve as reservoirs of extremophile microorganisms capable of surviving in extreme conditions. Objective: To determine the diversity of cultivable yeasts that colonize extreme domestic environments in the coastal region of Ecuador. Methodology: Samples were collected from biofilms, drains, and dish dryers, followed by primary isolation, which was cultured on Sabouraud Dextrose agar. Each isolate was characterized morphologically, physiologically, and biochemically. Before characterization, virulence factors were evaluated, and molecular techniques were used to identify the species to which it belongs. Results: Ten locations were selected, from which 28 microbial strain isolates were obtained. From these strains, 12 yeasts were identified, and various virulence factors were determined, including hemolysin, protease, and lipase. PCR amplification of the ITS region revealed that the most common strains belonged to the genera Candida metapsilosis and Kodamaea ohmeri. Conclusion: The prevalence of pathogenic yeasts in domestic environments is highlighted, which has implications for human health in the coastal region of Ecuador.
