Trabajos de Titulación - Medicina Veterinaria

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Análisis del efecto del extracto e la guacima (guazuma ulmifolia) en cepas certificadas de Salmonella Typhi
(Universidad Católica de Cuenca., 2021) Morocho Duy, Italo Adrian; Iñiguez Heredia, Italo Adrian; 0302126750
This research was aimed to evaluate the effect of guácima (Guazúma ulmifolia) extract and oil on Salmonella typhi ATCC 14028 ® strains, the raw material was collected in the community of Centropatul, the guácima extract was obtained by maceration, using guácima leaves. The oil was extracted from the guácima seed extracted from the dried ripe fruit, dried 3 days, and crushed, whit the help of Soxhlet equipment we separated the essential oil from the solid part, From 30 g of the sample we obtained 0.5 ml, its yield was 2.26 ml, which were placed in blank sensitivity discs, in different concentrations, (T1) guácima extract at 5%, (T2) guácima extract at 25%, (T3) guácima extract at 50% (T4) guácima extract at 100%, (T5) 100% guácima seed essential oil, was prepared by dilutions from the main extract, for the elaboration of the antibiogram, before this, Salmonella typhi ATCC 14028 ® was cultivated on SS agar, the it was sown on Mueller Hinton agar, the antibiogram process; based on the studies conducted, we were able to determine that the average oil obtained was 0.83 ml in 6 extractions, however, Salmonella typhi ATCC 14028 ® did not show sensitivity to the treatments. Keywords: Oil, Extract, Guácima, Salmonella, Typhi.