Caracterización morfométrica del semen colectado por masaje abdominal en líneas de gallos criollos

Abstract

The semen quality of creole roosters, collected by abdominal massage, was evaluated using 9 roosters of homogeneous weight and age belonging to three biotypes: Cubans, Barbons, and Guáricos. These had a training period of four weeks, which facilitated semen extraction. Semen collection was performed for 3 weeks, where five samples were extracted from each bird, these being the replicates. Each pool of genetic material was evaluated, regarding the variables color, mass, and individual progressive motility statistical differences were observed (P<0.05) in favor of the Cuban biotype with a qualitative value of 5 that corresponds to a milky white consistency and an observable motility of dense waves, fast and constant movements. For sperm volume and sperm concentration of the creole roosters, the analysis of variance did not show any significant differences (P>0.05). Vitality and viability were quantified by the Eosin Nigrosin method and the HOST method, where sperm vitality was similar (P>0.05) in the three biotypes, unlike viability (P<0.05), where Cuban roosters presented higher values with a 75% chance of fertilization. The variables were correlated, where it was estimated that sperm volume and sperm concentration increase simultaneously (P<0.05). It was concluded that the biotype with the best seminal quality is the Cuban, presenting better results in color, motility, and viability improving these values over time, this information is relevant for the conservation and genetic improvement of poultry.

Keywords: Seminal quality, abdominal massage, cubans, barbons, guaricos.