Propagación “in vitro” del penco bicolor (Agave americana) para la flora urbana de la ciudad de Cuenca

Abstract

The objective of this research was to evaluate the efficiency of in-vitro micropropagation techniques of penco bicolor (Agave americana), in which 120 seedlings were used, extracting the auxiliary buds. The explants introduced in vitro were incubated at 18.5°C for 21 days, using 4 replicates. During the rooting stage, 4 treatments were used, using Murashige and Skoog 4.4 g/l culture medium, added with 4 mg/l naphthaleneacetic acid (ANA) and 3 mg/l kinetin and 12 g/l agar for solidification. The results obtained determined that the best propagation protocol was through the evaluation of explants with shoots per treatment and the number of shoots per explant, obtaining the highest percentages of explants with shoots in treatments 2, 4 and 8 with values of 50%, 10% and 20%, respectively; On the other hand, treatments 10 and 1 showed 100% and 80% incidence of contamination, while treatments 1, 3, 5, 6, 7, 9 and 10 did not show explants with sprouts. In addition, the count of the number of sprouts per explant showed that the treatment with the highest number of sprouts was T2, while treatments T1, T3, T5, T6, T7, T9 and T10 did not show any sprouts. The explants were immersed in 20% tween for 10 min and rinsed. For disinfection, they were placed in 70% alcohol and 20% chlorine for 10 min, rinsed three times in sterile distilled water and then seeded in culture medium. Keywords: hormones, in-vitro, sprouts, fungi, bacteria