Efecto biocida del extracto de raíz del barbasco (lonchocarpus utilis)en el control de piojos en cuyes

Abstract

The study evaluated the biocidal effect of Cubé resin (Lonchocarpus utilis) for controlling lice in guinea pigs. A total of 180 improved-genotype guinea pigs in the growth phase (400 ± 50 g) were used, distributed across six treatments: T0 (control), T1 (25%), T2 (20%), T3 (15%), T4 (10%), and T5 (5%), with three replicates of 10 guinea pigs each. Cubé resin underwent collection, cleaning, drying, crushing, pulverization, and sieving. Its quality and toxicity were assessed through a bioassay with Artemia salina, determining an LC50 of 0.50% at three hours. The biocidal efficacy was tested in two phases. In the in vitro phase, 150 lice were exposed to five concentrations (T1, 100%; T2, 80%; T3, 60%; T4, 40%; and T5, 20%), with 10 lice per concentration. In the in vivo phase, infested guinea pigs were treated with Cubé resin at 25%, 20%, 15%, 10%, and 5% at a dose of 1% of their live weight, and lice mortality was evaluated at 6, 12, and 18 hours. The in vitro results showed significant differences (p<0.05), achieving the highest mortality (100%) at the 100% concentration after 18 hours. In the in vivo phase, all concentrations reached 100% mortality at 6 hours, whereas the commercial product achieved its maximum mortality at 12 hours. It is concluded that Cubé resin at concentrations below 25% has a biocidal effect and could serve as an alternative to commercial treatments.

Keywords Lonchocarpus utilis, Barbasco, Lice, Guinea pigs..