Identificación de cepas bacterianas fijadoras de nitrógeno dentro del cepario del CITT para su posterior experimentación en fabricación de biofertilizantes

Abstract

Several agricultural strategies, such as chemical fertilizers and pesticides, have been used to maximize yields. A sustainable alternative involves green technologies, such as biofertilizers based on soil microorganisms. The objective was to identify bacterial strains with nitrogen fixation capacity for a potential biofertilizer using biochemical techniques. A group of 67 strains was reactivated and analyzed to evaluate their potential. The microbiological study showed a predominance of flat colonies (81%), diversity in shapes, and bacilli (59%) among Gram-positive bacteria. It was also observed that irregular shapes predominated (30%), with white as the predominant bacterial color (43%), and medium-size strains as the most prevalent with 42%. Halo sizes between 20 and 10 mm show effectiveness in nitrogen fixation, with Acinetobacter Iwoffi and Providencia alcalifaciens highlighting. These results support the importance of these strains in sustainable agriculture and soil restoration.