Uso de semen sexado en un protocolo simplificado de fsh-p más ecg en vacas holstein en producción.

Abstract

Superovulation is reproductive biotechnology that has been widely used in cattle for several decades. However, applying several doses of pFSH to donors for four days causes stress in the animal. Recently, it has been shown that replacing the last four doses of pFSH (porcine Follicle Stimulating Hormone) with one dose of eCG (Equine Chorionic Gonadotropin) produces an equal number of embryos and a superovulation response similar to that of the conventional protocol. Therefore, this study’s objective was to use two hormonal protocols, such as pFSH and a combination of pFSH plus eCG. Treatments were applied employing the FTAI technique with sexed semen. These studies were conducted on 4 Holstein Friesian female cattle in production, about 90 days postpartum, with a body condition of 2.75 to 3. They are located in the Pilatos sector of the Biblián canton, in the province of Cañar. Embryo collection was performed seven days after insemination using sexed semen once the treatment was completed. The superovulation response resulted in the number of corpora lutea and viable embryos from both the first and second treatments showed no significant difference. Therefore, it can be concluded that the simplified protocol of pFSH plus eCG produces a superovulation response and a similar number of transferable embryos when using sexed semen compared to the conventional protocol.

Keywords: Holstein, cattle, superovulation, pFSH, eCG, sexed semen.