Inducción hormonal (BAP-ANA) de brotes en plantines in vitro de Phalaenopsis bajo dos espectros de luz y dos concentraciones de Murashige Skoog

Abstract

The objective of the study was to evaluate the concentrations of the hormones BAP (6-Benzylaminopurine) and NAA (1-naphthaleneacetic acid), light spectra and the doses of Murashige and Skoog (1962), which favor shoot development in Phalaenopsis seedlings in vitro. Three concentrations of hormones H1 (BAP 0 mg/l + NAA 0 mg/l), H2 (BAP 3 mg/l + NAA 0.5 mg/l) and H3 (BAP 6 mg/l + NAA 0.5 mg/l) were used; two types of light spectra WL (white light) and BRL (blue-red light) were tested; accompanied by two Murashige and Skoog doses (100% MS and 50% MS). Twelve treatments were obtained, evaluated 15 days after planting, and monitored every eight days for two months. The results obtained in the research show that the treatments (T3, T4, T5, T6, T9, T10, T11, and T12) with growth regulators presented shoot induction in Phalaenopsis seedlings compared to the treatments that did not have growth regulators and that did not present shoot induction (T1, T2, T7 and T8). The combination of growth regulators BAP 6mg/l + NAA 0.5 mg/l showed more sprouts. As for the culture media, Murashige and Skoog showed no significant difference in seedling induction. The white light spectrum significantly differed in shoot induction compared to the red-blue light spectrum.

Keywords: Naphthaleneacetic acid (NAA); benzyl aminopurine (BAP); in vitro bud induction; Phalaenopsis. .