Evaluación del efecto in vitro del extracto de altamisa (ambrosia sp) sobre piojos (haematopinus sp)

Abstract

This research work aims to obtain the extract of the plant (Ambrosia spp), in which two different methods of extraction will be used, Maceration and Soxhlet, from which the best performance of the plant in each of them will be evaluated, as well as its effectiveness in the in vitro application on lice (Haematopinus spp). This study is of an explanatory type with an experimental design consisting of subjecting groups of lice separated in experimental units to certain treatments at different doses proposed five times to observe the effects or reactions that occur in their mortality. Thus, after applying the extract of Altamisa (Ambrosia spp). It will be seen are the yields in the quantities used of each plant and the effectiveness of the extract in the doses proposed, the times used in obtaining and treating the plant, in addition to the time taken by each extraction method in the laboratory process. A better yield was obtained with maceration with 3.36%, compared to the Soxhlet method with only 2.3% of viable active principle for the experiment. In which Soxhlet had a better LD50 in the concentration of 9.4% in a TL50 of 3.05 hours, while with maceration an LD50 of 10.4% was obtained with a TL50 of 3.05 hours. This gives meaning to all the research proposed, in addition, and mainly, it is a viable and low-cost way for all those small producers who are looking for an economic and accessible way to treat their animals without causing resistance or toxicity.

Keywords: Altamisa, extract, maceration, head lice, soxhlet