Comparación de dos crioprotectores en la congelación de espermatozoides de Jambato del Azuay

Abstract

The decline of the amphibian population is becoming increasingly relevant since 44.8% of Ecuadorian amphibian species (164) are in danger of extinction. Therefore, a focus should be made on the cryopreservation of amphibians to counteract the loss of their genetics using assisted reproductive techniques. The current research shows the results of comparing two cryoprotectants, Dimethyl sulfoxide (DMSO) and Dimethylacetamide (DMA), in the freezing of spermatozoa of Atelopus bomolochos. A concentration of 10% was used for freezing in both treatments. The results for the first variable assessing abnormalities showed ineffectiveness in protecting sperm quality for both treatments. Specifically, DMSO had a 47.5% ineffectiveness rate, while DMA had a 31.5%. Regarding plasma membrane integrity, DMSO obtained a percentage of 76.25% versus 100% for DMA. Both DMSO and DMA showed negative results regarding mitochondrial functionality, with 100% of the spermatozoa being non-functional for both treatments. Keywords: Dimethyl sulfoxide, Dimethylacetamide, Mitochondrial functionality, Morphology, Plasma membrane integrity, Anuros, Atelopus.