Evaluación de la crioconservación espermática en líneas de gallos criollos con glicerol al 8% como alternativa para el mejoramiento genético.

Abstract

Cryopreservation of creole rooster semen represents a reproductive technique as an alternative for genetic resources to reduce the risk of extinction, keeping the sperm frozen and preserving its viability. The sperm quality of creole rooster semen was evaluated with cryopreserved 8% glycerol, collected by dorsal abdominal massage, diluted with Lake-Ravie to be frozen in 0.25 ml straws using two liquid nitrogen ramps and thawed at 5 °C. The present investigation was carried out in the laboratories of the Academic Unit of Agricultural Sciences of the Catholic University of Cuenca. The statistical method used was a completely randomized block design using 3 treatments with 5 replicates, for which 9 roosters of homogeneous weight and age belonging to three biotypes: Cuban, bearded, and guaricos, were used for 3 weeks. When evaluating the progressive mass and individual motility in fresh and post-thawed semen, significant statistical differences were obtained (p≤0.05) in favor of the Cuban biotype. Regarding the HOST method, we obtained statistical differences in the evaluation of fresh and thawed semen (p≤0.05), for the sperm vitality test we used the eosin - nigrosa technique, obtaining statistically different values in the thawing stage and not significant in the evaluation of fresh semen. The application of 8% glycerol is a sufficient technique to preserve poultry semen and the optimal protocol for them is the previous training in the rooster lines. Keywords: seminal quality, mass motility, straws, sperm viability.