Efecto de la concentración de la coenzima Q10 en la refrigeración de semen ovino

Abstract

The sperm cryopreservation process faces considerable challenges, especially regarding the integrity of the plasma membrane, which becomes more fragile with refrigeration and freezing, affecting both the morphology and function of the sperm. In this study, the effect of various concentrations of Coenzyme Q10 (0.2 mM, 0.4 mM, 0.6 Mm, and 0.8 mM) on the quality of sperm from sheep of the Katahdin, Dorper, and Pelibuey breeds after different periods of refrigerated storage (0 h, 24 h, and 48 h) was investigated. The research conducted at the Catholic University of Cuenca for five months used techniques such as sperm motility analysis, vitality tests, and evaluation of mitochondrial activity using Rhodamine 123 and Propidium Iodide. The results indicated that different concentrations of Coenzyme Q10 had a significant impact on sperm quality. The 0.8 mM concentration showed the best results regarding motility, vitality, and mitochondrial activity, while lower concentrations showed a decrease in these parameters compared to the control group. Furthermore, it was observed that this coenzyme reduced the formation of crystals during refrigeration, improving sperm quality, especially at 0.6 mM and 0.8 mM. Katahdin rams exhibited superior initial sperm quality, which benefited from subsequent extractions. Keywords: Coenzyme Q10, Refrigeration, Sperm Quality.