Calidad funcional y morfometría de espermatozoides de zángano expuestos a glifosato.

Abstract

The objective of the study was to evaluate the effect of exposure to glyphosate for agricultural use under controlled environments on the kinetics and morphometry of spermatozoa from drones from hives installed above 2,200 m.a.s.l. For this purpose, 16 drones from different locations were used: Tarqui, Paute, and Cuenca. The treatments to be evaluated were dose 1 (0.25%), dose 2 (0.15%), dose 3 (0.10%) and dose 4 (0.05%) of glyphosate. The variables studied were the drone's time of death, flight, walking, stopping, and sperm morphology (length, width, head area, perimeter, elongation, roughness, regularity, volume, concentration, vitality, and death). The results obtained show that the location of the hives about the application of glyphosate does not affect the variables analyzed. However, drone’s time of death and time of stopping were affected under 0.25, 0.15, 0.10, and 0.05% doses; in the same way, flight time and walking time were significantly different under amounts of 0.25 and 0.05% of glyphosate. Finally, the sperm morphology of the drones was quite different, being affected by the parameters of sperm vitality and sperm death. The conclusion is that the use of glyphosate in agricultural production helps control undergrowth; however, it negatively affects the environment, in this case affecting beneficial insects (drones), causing problems in the reproductive part and, in more severe cases causing their death. Keywords: sperm morphology, glyphosate, drone, kinetics.