Evaluación del efecto de cuatro combinaciones hormonales, dos concentraciones de medio en la micropropagación in vitro de tres cultivares de anturio (anthurium andreanum)

Abstract

Anthurium andreanum is an ornamental plant of great popularity and acceptance in the floricultural sector due to the shape of its foliage and showy flowers. Unfortunately, conventional sexual and asexual propagation methods are not practical to achieve massive multiplication of this species. Therefore, this research aimed to evaluate the effect of four hormonal combinations, two concentrations of Murashige and Skoog (MS) culture medium, on the in vitro micropropagation of White, Pink, and Orange A. andreanum cultivar (cv.). In consequence, two protocols for disinfection of explants from leaves were tested; the first treatment constituted of 70% Ethanol for 30sec, 0.5% NaClO for 15 min; the second treatment was 70% Ethanol for 1 min, 1 and 0.5% NaClO, for 5 and 15 min respectively. The result was that disinfection protocol number one presented tolerable levels of contamination (20.04%) and browning (36.33%) compared to protocol number two, which showed exuberant levels of browning (97.11%). The optimal culture medium for the micropropagation of A. andreanum of explants from leaves was 50% MS supplemented with 0.5 mg/L−1 of TDZ and 0.2 mg/L−1 of 2-4 D. The Pink cv. exhibited 90% callus formation and 8.5 shoots/explant, the Orange cv. showed 88.3% callus and 3 shoots/explant, and finally, the White cv. only presented 2% callus formation. In future research, it is recommended to test low concentrations of Kinetin and Naphthaleneacetic Acid in White cv.