Producción de embriones in vitr de vacas Holstein estimuladas en fsh-rb

Abstract

The conventional use of FSH (follicle-stimulating hormone) in follicular hyperstimulation has raised concerns due to its effects on gene expression and embryo quality. In addition, considerable variability in the response of hyperstimulated cows has been observed, affecting the efficacy of the process. Follicle Stimulating Hormone - pituitary (FSH-p) of porcine origin, commonly used in these protocols, can induce immune responses in cattle, increasing the risk of pathologies and affecting the quality of the resulting embryos. In contrast, recombinant bovine Follicle Stimulating Hormone (rbFSH), derived from recombinant technology, is a promising alternative to improve in vitro embryo production and quality in Holstein cows. This research aimed to thoroughly evaluate the effects of rbFSH on in vitro embryo production in Holstein cows. The results indicate that rbFSH did not significantly increase the production of viable oocytes according to the classification table established by the International Embryo Technology Society (IETS). On the other hand, during embryo development, the stage of segmentation was evaluated, a percentage variable measured as cleavage rate, where it is clear to note a specific numerical difference in the group treated with bovine recombinant FSH (74.38) compared to the control (68.4), but this difference was not statistically significant (P<0.05), obtaining similar statistical results in terms of the number of embryos obtained at the end of this study.

Keywords. Production; embryos; rbFSH; culture; In vitro.