“Análisis y determinación de microorganismos bacterianos presentes en el Hospital Humanitario fundación Pablo Jaramillo”

Abstract

Introduction: Nosocomial infections, also known as intrahospital infections, are caused by opportunistic bacteria commonly found in patients suffering from specific pathologies —infections associated with patient care within health facilities—. Objective: To determine the presence of pathogenic microorganisms on inert and environmental surfaces in the Intensive Care Unit (ICU), Neonatology, and Emergency Departments of the "Pablo Jaramillo" Foundation - Humanitarian Hospital (Hospital Humanitario - Fundación Pablo Jaramillo).

Methodology: This was basic research with a positivist paradigm using a quantitative, in vitro experimental approach. Surface and intrahospital environmental analyses were conducted. The surface swab method was applied for sampling in the neonatal, ICU, and emergency departments, focusing on high-contact areas. The presence or absence of each type of pathogenic microorganism was determined by colony staining on the Compact Dry plates.

Results: A total of 75 samples were analyzed, and it was demonstrated that E. coli was present in 21.05% of the samples, S. aureus in 60.00%, Klebsiella spp. in 37.50%, molds and yeasts in 30.00% and were absent in 70.00% of the cases. Conclusion: The most frequent encountered pathogenic microorganisms were Klebsiella spp and S. aureus on the inert surfaces. This suggests that hygiene and cleaning regulations are being followed, thus reinforcing disinfection practices in the analyzed areas.