Bioquímica y Farmacia
URI permanente para esta comunidadhttps://dspace.ucacue.edu.ec/handle/ucacue/26
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Examinando Bioquímica y Farmacia por Asesores "Yarzábal Rodríguez, Luis Andrés"
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Ítem Acceso Abierto Caracterización fenotípica y ubicación filogenética de cepas de levaduras del género Rhodotorula y afines en ambientes domésticos de las diferentes regiones del Ecuador(Universidad Católica de Cuenca., 2025) Segarra Espinoza , Juan Carlos; Vásquez Díaz, Mario Esteban; Yarzábal Rodríguez, Luis Andrés; 0150565190; 0106990757Introduction: The growing interest in environmental microbiology has highlighted the importance of studying the complex diversity of microorganisms inhabiting domestic spaces. These environments, often overlooked in microbiological studies, harbor microbial communities whose presence can have significant and direct implications for the health of the inhabitants. Objective: To characterize yeast strains at the phenotypic and molecular levels belonging to the genus Rhodotorula and related species that colonize extreme domestic environments in different regions of Ecuador. Methodology: This study was conducted using an experimental and descriptive research design. Quantitative and qualitative approaches were used to phenotypically and genetically characterize the yeast strains of the genus Rhodotorula. Results: Primary isolation of different strains of the genus Rhodotorula was performed from domestic environments, specifically from kitchen sinks in several households in different regions of Ecuador. Conclusion: The data obtained in the present study support that kitchen sinks in domestic environments can be colonized by different genus Rhodotorula and Cytobasidium species, and these strains show affinity for certain virulence factors.Ítem Acceso Abierto Diseño, evaluación y optimización de estuches didácticos para el fortalecimiento de la enseñanza de microbiología y biotecnología(Universidad Católica de Cuenca., 2025) Vásquez Espinoza, Pablo Andrés; Méndez Andrade, Israel Sebastián; Yarzábal Rodríguez, Luis Andrés; 1401041445; 0107432577ntroduction: Scientific and technological development requires highly skilled professionals. However, conventional theoretical classes present limitations in the teaching of natural sciences, leading to disinterest and learning difficulties in students. In this study, didactic kits were designed and evaluated for academic performance and the understanding of fundamental concepts through scientific practice. Objective: To design, optimize, and evaluate the pedagogical capacity of didactic kits. Methodology: A literature search was conducted using Boolean operators in databases. Subsequently, experimental protocols were designed for the implementation of the kits. Their pedagogical effectiveness was evaluated through a pilot test with first-year Biochemistry and Pharmacy students at the Catholic University of Cuenca. Results: The post-test results showed a better performance in the experimental group, indicating statistical significance for most parameters. Therefore, the students in the experimental group improved after using the didactic kits. Conclusion: The didactic kits strengthen academic learning in the early years of the Biochemistry and Pharmacy program. Furthermore, the use of these kits stimulates the acquisition of novel theoretical knowledge, and contributes to a pedagogical and academic improvement Keywords: Didactic kits, methodology, teaching, scienceÍtem Acceso Abierto Extremófilos en ambientes antropizados: Aislamiento de bacterias halotolerantes a partir de sal doméstica(Universidad Católica de Cuenca., 2025) Gómez Minchalo, Andrea Silvana; Morales Ulloa, Nicole Michelle; Yarzábal Rodríguez, Luis Andrés; 0150625424; 0605446863Introduction: Saline environments are home to extremophilic microorganisms, especially halophilic and halotolerant bacteria with biotechnological potential, thanks to their enzymes and metabolites. To isolate them, products from these environments can be used, such as unprocessed table salt. Objective: To detect the presence of viable and culturable microorganisms in table salt samples available in anthropized spaces (supermarkets). Methodology: Bacteria were isolated from table salt samples using Sabouraud Dextrose Agar and Nutrient Agar supplemented with NaCl. The isolates were morphologically characterized, and their halotolerance was evaluated. Molecular identification was performed by sequencing the 16S rRNA gene and conducting subsequent bioinformatic analysis. Results: Halotolerant microorganisms were detected in samples of Flor de Sal and smoked salt, with greater diversity observed in the former (13 morphotypes) compared to the latter (4 morphotypes). Seventeen isolates were purified, with 11 selected for further studies. The majority were Gram-negative bacilli, and yeasts and cocci were also detected. All isolates exhibited tolerance to NaCl concentrations ranging from 0.5% to 10.5% (w/v), but none grew at 20.5%. Bioinformatic analysis identified strains related to the species Priestia flexa, Bacillus boroniphilus, Bacillus aquimaris, Cytobacillus pseudoceanisediminis, Mesobacillus maritimus, Aureimonas altamirensis, Pantoea eucrina, and Kocuria rhizophila. Conclusion: Eight halotolerant isolates were identified, showcasing their ability to thrive at varying NaCl concentrations. Some strains exhibited biotechnological potential, such as the production of substantial exopolysaccharides. These findings emphasize the presence of significant microbial diversity in table salt and its relevance for biotechnological applications.
