Trabajos de Titulación - Medicina Veterinaria
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- ÍtemAcceso AbiertoValoración de un esfigmomanómetro aneroide de presión modificada para la insuflación del balón de neumatoponamiento del tubo endotraqueal en perros(Universidad Católica de Cuenca., 2021) Muela Carreño , Kerly de los Angeles; Castillo Hidalgo, Edy Paúl; 0302478581This paper entitled "Evaluation of a modified aneroid pressure sphygmomanometer for the insufflation of the endotracheal tube cuff balloon in dogs" was developed to conduct a study to determine the exact values for the injection of air into the endotracheal cuff balloon. This study was carried out at the facilities of the "Le Peluts" veterinary clinic. The endotracheal tube with low pressure and high volume cuff was developed with the help of a three-way key connecting one end of the key to the sphygmomanometer, the other end to the pilot balloon and the last key is connected to a syringe. The first method that was performed is the technique of digital-palpation where the amount of air that is sent to the endotracheal cuff balloon was not measured, the second technique was with the sphygmomanometer in which it was possible to measure the air pressure that was sent to the endotracheal cuff balloon and it is there where it is verified which of the two techniques is the most feasible to avoid pathologies that occur during and after intubation with the endotracheal tube. No physical changes were observed in the samples taken using the sphygmomanometer. In the second method, which is the digital-pressure technique, morphological changes were observed, that is to say, that there was irritation, superficial and deep ulcers in the patient. KEYWORDS: ENDOTRACHEAL, SPHYGMOMANOMETER, INSUFFLATION, ANEROID, ENDOTRACHEAL INTUBATION PROCEDURE
- ÍtemAcceso AbiertoReemplazo parcial de la hormona folículo estimulante porcina (FSH-p)por gonadotrofia coriónica equina (eCG) en la superovulación de vacas en producción(Universidad Católica de Cuenca., 2021) Sánchez Sánchez , Luis Miguel; Argudo Garzón , Daniel; 0105566384Milk production is of great importance to the national economy. Through superovulation and embryo transfer it is possible to obtain genetically improved animals, and therefore improve the productivity of dairy farms. The objective of the present study was to evaluate the effect of substituting the last four doses of FSH in the superovulation protocol for two doses of eCG in dairy cows. Eight Holstein cows were subjected to a protocol of follicular wave and ovulation synchronization, and ovarian stimulation. Cows were randomly assigned to one of the following two superovulation treatments: FSH, 8 decreasing doses of FSH for 4 days; FSH + eCG, the last four doses of FSH in the conventional protocol were replaced by a 600 IU dose of eCG. The data were analyzed by analysis of variance of the SPSS statistical program. The ovulatory response was similar between treatments (10.25 ± 1.31 and 10.00 ± 1.29 for FSH and FSH + eCG respectively). The cows treated with FSH (6.00 ± 0.58) had a higher number (P0.05) of anovulatory follicles than the FSH + eCG (3.25 ± 0.63). The number of recovered structures, transferable embryos, degenerated embryos and unfertilized oocytes were similar between treatments. In cows treated with FSH + eCG, more morulae (45.6 vs 20.1% respectively) and expanded blastocysts (6.8 vs 0.0% respectively) and fewer regular blastocysts (40.7 vs 6.6% respectively) were recovered than FSH treatment, although differences were not significant. Cows treated with FSH produced fewer quality 1 and more quality 2 blastocysts than FSH + eCG treated cows. In conclusion, substituting the last four doses of FSH in the conventional superovulation protocol for one dose of eCG did not improve the superovulatory response or the production of transferable embryos. Keywords: multiovulation, embryo transfer, FSH, eCG, dairy cows.
- ÍtemAcceso AbiertoFactores de riesgo en enfermedades periodontales con prevalencia de pasteurella multocida, en el refugio "Arca"(Universidad Católica de Cuenca., 2024) Pachar Pachar, Paulina Elizabeth; Aguilar Caivinagua, Santiago; 0107938409P. multocitica is one of the most common bacteria found in the oral cavity of domestic animals (dogs and cats). This study considered 50 canines housed at the "Arca" shelter in Cuenca, Ecuador, under similar nutritional (homemade food - pelletized) and environmental conditions. This study was influenced by several external factors beyond those mentioned earlier. The colonization of the P. multocitica bacteria can also be influenced by age, gender, and oral health status. Specifically, if there is a pre-existing gum condition where plaque accumulates, it becomes an ideal environment for this bacterium. Of the 50 gingival samples, 24 canines with P. multocitica were identified. From this point on, all external factors are analyzed. They were divided into two groups based on age. The first group, consisting of puppies (0-24 months), presented 5 cases of P. multocitica. On the other hand, the group of adult dogs (3-7 years old) showed 19 cases. Regarding gender, 30% was represented by males and 18% by females, resulting in a higher affinity of this bacterium for males; however, these data are statistically not very significant. Finally, across the four stages of periodontal disease, differential data was observed in each group, with stage IV showing a higher bacterial load than the baseline group, where a statistical difference was noted (p<0.05). Keywords: P. multocitica, age, gender, periodontal disease
- ÍtemAcceso AbiertoEfecto de la concentración de la coenzima Q10 en semen ovino post congelación(Universidad Católica de Cuenca., 2024) Loja Jimenez, Evelyn Thayni; Moscoso Piedra, Andres Leonardo; 1900857564Reproductive biotechnologies are essential in sheep genetic improvement processes to increase productive performance. These tools address problems of species infertility and productivity. In this scenario, coenzyme Q10 (ubiquinone) is a substance that plays a fundamental role in semen cryopreservation, acting as an antioxidant in sperm survival. In this research, the effect of coenzyme Q10 concentration in post-freezing sheep semen was evaluated, with different doses (0.2mM, 0.4mM, 0.6mM, and 0.8mM). In consequence, samples of three phenotypes were extracted, with five repetitions, dividing each sample into four doses plus one control. The samples were contrasted with the fresh samples that were previously evaluated, and where the Katahdin individual was the one that showed the best characteristics. However, when adding coenzyme Q10, its effect on the thawed samples was not encouraging and was evidenced by a significant reduction (p<0.05) in the motility indicators, defined by the CASA system (Computer Assisted Sperm Analysis System); this reduction was associated with the presence of coenzyme Q10 in the frozen medium. The membrane functionality and viability tests determined a reduction of these indicators associated with the concentration of coenzyme Q10. A more exhaustive investigation is required on this response of coenzyme Q10 to justify its lethal and sublethal effect due to the lack of specific literature about it; thus, it is necessary to use lower doses to validate this statement by contrasting it with other freezing methodologies.
- ÍtemAcceso AbiertoCambios en la composición microbiana de quesos semimaduros en función del cuajo utilizado(Universidad Católica de Cuenca., 2024) Yanza Contreras, Luis Eduardo; Iñiguez Heredia , Franklin Alfredo; 0104904644The use of rennet in cheese making is a practice in constant evolution, being animal rennet a millenary technique of great cultural and historical value despite the hygienic risks that this entails. This quasi-experimental observational study focused on investigating the details of the process of making semi-mature cheeses according to the rennet used and determining its effect on the microbial composition in the ripening of cheeses for four weeks. It was conducted in the laboratories of the Academic Department of Agricultural Sciences Faculty of the Catholic University of Cuenca. The research compared three treatments with the same milk using different types of rennet (lactating goat, microbial, and synthetic). Ten cheeses were made per treatment, with each cheese representing one experimental unit; their organoleptic characteristics and microbial load were analyzed to determine significant treatment differences. In terms of organoleptic properties, no differences were found between treatments; however, there were differences in the microbial load between treatments. On the one hand, it was observed that there are fewer enterobacteria in the goat cheese —which should be further studied in future research— and a higher concentration of Lactobacillus in the cheeses made with animal and microbial rennet compared to synthetic rennet. This suggests that these rennets are more effective in providing probiotics beneficial to intestinal health, which could have significant implications for the nutritional quality of cheeses.
