Agronomía
URI permanente para esta comunidadhttps://dspace.ucacue.edu.ec/handle/ucacue/54
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Examinando Agronomía por Materia "análisis flujo retrogrado"
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Ítem Acceso Abierto Relación de las caracteristicas corporales, morfometría testicular y la viabilidad de espermatozoides epididimarios en caninos post-orquiectomia(Universidad Católica de Cuenca., 2023) Jiménez Jaramillo, Erik Mauricio; Veloz Veloz, Dina Marisela; 1105116949Studying the relationship between testicular morphometry, age, weight, and sperm viability in dogs is fundamental to understanding animal reproduction. This study aimed to correlate body characteristics, testicular morphometry, and post- orchiectonomy epididymal sperm viability. The clinical records of the patients were obtained, 26 testicular samples were collected, the samples were transported in sterile containers with 9% NaCl solution, and testicular morphometric data such as length, depth, width, and weight were recorded. Spermatozoa were collected using the retrograde flow technique with Tris+yolk solution. Each sample was evaluated to determine sperm quality, and the parameters assessed were: concentration, individual progressive motility, vitality, plasma membrane, and acrosomal integrity. A correlation test was performed between testicular morphometry and sperm kinetics, finding a moderate correlation between these two variables, with testicular length being one of the most relevant referents. Age and weight were also related to sperm parameters, with age showing a positive relationship with sperm concentration but a negative tendency with the fluorescence test. Weight showed a high relationship with individual progressive motility and hypo-osmotic test. Overall, the results indicate that the body characteristics of dogs can influence their sperm quality, highlighting the importance of understanding the relationship between these characteristics and sperm quality in dogs, which may have implications for animal reproduction and breeding protocols. Keywords: correlation, epididymis, morphometry, analysis, retrograde flow
