Examinando por Autor "Verdugo Morales, Ivana Sofia"
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- ÍtemAcceso AbiertoRelación de las Características corporales y morfmetria testicular en la criopreservación de espermatozoides epidimarios caninos(Universidad Católica de Cuenca., 2023) Verdugo Morales, Ivana Sofia; Villacis Cabrera, Daniela Estefanía; Veloz Veloz, Dina Marisela; 1729159523; 0105744981This study aimed to determine the relationship between body characteristics and testicular morphometry with the qualitative and kinetic components of sperm obtained from the epididymis tail of dogs before and after cryopreservation following orchiectomy. Testicular samples were obtained from 26 dogs of different ages. The testes were placed in saline solution (0.9%) and refrigerated for 24 hours before freezing. Spermatozoa were recovered by retrograde flow technique and Tris-egg yolk dilution. Glycerol at 5% was added as a cryoprotectant for cryopreservation. The sample was packed in 0.5 ml straws and subjected to a cooling curve for two hours at 5 ºC, then placed over liquid nitrogen vapors for 15 minutes and subsequently immersed directly. The parameters evaluated were: progressive individual motility, plasma membrane integrity, vitality, and membrane and acrosome morpho-anomalies before and after freezing. Additionally, concentration data were obtained prior to freezing. The parameters were drastically affected after freezing, leading to the conclusion that external factors and sample handling can affect the sperm quality of epididymal semen in dogs. This study found more positive relationships, indicating that sperm quality is qualitatively higher in medium-sized animals compared to small and large ones. Therefore, it is necessary to identify these factors in future research. Keywords: testes, cryopreservation, dogs, testicular morphometry