Examinando por Autor "Valdez Pinoargote, Girabel Gissela"
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Ítem Acceso Abierto Efecto de la concentración de la L-carnitina en congelación del semen ovino(Universidad Católica de Cuenca., 2025) Valdez Pinoargote, Girabel Gissela; Moscoso Piedra, Andrés Leonardo; 0804298040Sperm cryopreservation is a key technique in animal reproduction, particularly in ruminants, enabling assisted reproduction and benefiting veterinary medicine. L-Carnitine, an amino acid derived from lysine and methionine, plays a crucial role in preserving membrane integrity and mitochondrial function. The aim of this study was to assess the effect of Lcarnitine supplementation at doses of 1M, 0.750mM, 0.500mM, and 0.100mM on the sperm quality of three sheep breeds (Dorper, Kathadin, and Pelibuey). Semen was collected from each sheep over multiple mounts, resulting in three ejaculates per animal, with a total of nine samples, and a control test with semen free of L-carnitine. These samples were evaluated through laboratory tests (Eosin-Nigrosin, Propidium Iodide, and Rodamine) and analyzed using the CASA system to measure parameters such as PR, NP, IM, VCL, VAP, VSL, STR, LIN, ALH, and BCF. The results favored the 1M dose (highest concentration) across permeability, viability, and kinetics tests. Statistical differences were found in the LIN and STR values, consistently favoring the 1M concentration compared to the control. Keywords: Cryopreservation; L-Carnitine; Sheep; Spermatozoa
