Examinando por Autor "Coronel Sarmiento, Helen Nicole"
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- ÍtemAcceso AbiertoAplicación de dos técnicas de estimulación hormonal para extracción espermática en atelopus nanay, atelopus bomolochos y ctenophryne aequatorialis(Universidad Católica de Cuenca., 2023) Coronel Sarmiento, Helen Nicole; Zhumi Cajamarca, Jefferson Mauricio; Argudo Garzón, Daniel Ernesto; 0350191862; 0150765733Ecuador is recognized for its great diversity of amphibian species currently threatened worldwide. In this context, this study evaluated the feasibility of two hormonal techniques (GnRH and hCG) to improve sperm performance in three species: Atelopus nanay, Atelopus bomolochos, and Ctenophryne aequatorialis, in the Azuay province. The application of hormones is effective in stimulating sperm release in amphibians. Previous studies have obtained favorable results using GnRH and hCG in different species. The conservation of amphibians under human care has become crucial due to the decline of in situ populations. Currently, there is an amphibian conservation center of the Amaru Foundation in Cuenca, Ecuador. Sperm collection was conducted in three species of amphibians with hormone doses of 4 μg/g and five μg/g, respectively. The results show significant differences in sperm parameters between species and hormone treatments applied. It was demonstrated that the application of GnRH and hCG generated a high motility index between 3.5- and 5.5-hours post-application and a high sperm concentration between 2.5- and 3.5-hours post-application of the treatments in Atelopus nanay and Atelopus bomolochos. No relevant results were obtained for the species Ctenophryne aequatorialis.