Unidad Académica de Ciencias Agropecuarias
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Examinando Unidad Académica de Ciencias Agropecuarias por Asesores "Argudo Garzón, Daniel Ernesto"
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- ÍtemAcceso AbiertoAplicación de dos técnicas de estimulación hormonal para extracción espermática en atelopus nanay, atelopus bomolochos y ctenophryne aequatorialis(Universidad Católica de Cuenca., 2023) Coronel Sarmiento, Helen Nicole; Zhumi Cajamarca, Jefferson Mauricio; Argudo Garzón, Daniel Ernesto; 0350191862; 0150765733Ecuador is recognized for its great diversity of amphibian species currently threatened worldwide. In this context, this study evaluated the feasibility of two hormonal techniques (GnRH and hCG) to improve sperm performance in three species: Atelopus nanay, Atelopus bomolochos, and Ctenophryne aequatorialis, in the Azuay province. The application of hormones is effective in stimulating sperm release in amphibians. Previous studies have obtained favorable results using GnRH and hCG in different species. The conservation of amphibians under human care has become crucial due to the decline of in situ populations. Currently, there is an amphibian conservation center of the Amaru Foundation in Cuenca, Ecuador. Sperm collection was conducted in three species of amphibians with hormone doses of 4 μg/g and five μg/g, respectively. The results show significant differences in sperm parameters between species and hormone treatments applied. It was demonstrated that the application of GnRH and hCG generated a high motility index between 3.5- and 5.5-hours post-application and a high sperm concentration between 2.5- and 3.5-hours post-application of the treatments in Atelopus nanay and Atelopus bomolochos. No relevant results were obtained for the species Ctenophryne aequatorialis.
- ÍtemAcceso AbiertoCaracterización de semen de zángano y efecto de la jalea real en la crio preservación de los espermatozoides(Universidad Católica de Cuenca., 2023) Enríquez Duma, Carlos Fernando; Lalvay Quezada, Jonathan Santiago; Argudo Garzón, Daniel Ernesto; 0105623466; 0105936900Pollinator populations, particularly bees, have significantly declined due to environmental changes and the indiscriminate use of pesticides, affecting the number of hives worldwide. This has decreased their pollination service, triggering a low agricultural yield that jeopardizes food security. Cryopreservation offers a potential solution for species preservation. Therefore, this study aims to evaluate the effect of adding royal jelly to the cryopreservation of drone spermatozoa, for which seminal samples were extracted from 396 drones. Initially, the fresh semen was characterized as a starting point; then two treatments were prepared; T1 DMSO as a cryoprotectant along with a TCG-based diluent (tris, citric acid, glucose) containing egg yolk supplemented with 1% jelly, and T2 without jelly supplementation (control). These samples were cryopreserved to evaluate seminal quality after thawing. The results obtained indicated a 38.4% sperm collection efficiency and an average ejaculate volume of 0.8 µL, with a sperm concentration of 2.8 ± 0.43 X 106. In addition, significance for the morphology variable was expressed, while the motility, membrane integrity, and viability variables showed no significant differences. The data were subjected to the Student’s T-test. In conclusion, the post-cryopreservation data of drone semen revealed limited benefits; however, experimentation with the inclusion of percentages other than those used in this study is necessary to optimize the results