Unidad Académica de Ciencias Agropecuarias
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Examinando Unidad Académica de Ciencias Agropecuarias por Asesores "Alvarado Alvarado, Juan Carlos"
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- ÍtemAcceso AbiertoEfecto de la progesterona más estradiol sobre la sincronización, ovulación y calidad de embriones en yeguas de la raza árabe(Universidad Católica de Cuenca., 2024) López Espinoza, Walter Andrés; Alvarado Alvarado, Juan Carlos; 0302655873An intravaginal device containing progesterone (P4) plus estradiol benzoate (E2) was inserted into 10 Arabian mares to synchronize estrus and ovulation and evaluate the quantity and quality of embryos. A 1g P4 device and 2 mg of estradiol benzoate were inserted on day zero. On day 10, the device was removed, and 150 µg of sodium cloprostenol was administered. It was compared with a control treatment where cloprostenol at the same dose plus human chorionic gonadotropin (HCG) was administered on day 10. Using the device with estradiol and a luteinizing hormone proved effective in inducing ovulation in mares. On day 15, follicular monitoring determined that the size of the preovulatory follicle was >39 mm. The size of the follicle and corpus luteum was observed, with the follicle being more prominent in the primary treatment, with an average ovulatory follicle size of 41.55 mm compared to 40.8 mm in the control. The mean size of the corpus luteum was 30.92 mm in the primary treatment versus 29.7 mm in the control. However, no statistical difference between variables was observed, but there was a 40% correlation between follicle size and corpus luteum diameter. Follicular and corpus luteum irrigation was higher in the P4 treatment at 75%, compared to 38% in the prostaglandin treatment. Higher quality and quantity of structures were observed in the P4 treatment, reflecting a p-value of (p=0.55), indicating a statistical difference between variables by treatment.
- ÍtemAcceso AbiertoEstimulacíon ovárica en ovejas con la aplicación de FSH-p epidural en la producción in vitro de embriones(Universidad Católica de Cuenca., 2023) Pacheco Pacheco, Carlos Julio; Alvarado Alvarado, Juan Carlos; 0104287040This research aimed to assess the effects of ovarian stimulation for oocyte recovery using the Ovum Pick-Up (OPU) follicular aspiration technique with epidural administration of FSH-p compared to the combination of FSH-p and eCG administered intramuscularly in vitro embryo production. These three treatments were used: T1: 100mg of FSH-p epidurally divided into two doses (7:00 am-6:00 pm), combined with 300IU of eCG intramuscularly (IM). T2: 100mg of FSH-p (IM) divided into two doses (7:00 am-6:00 pm) plus 300IU of eCG (IM). T3: 300IU of eCG intramuscularly. The variables measured were: Cumulus cell expansion, maturation percentage, fertilization percentage, and embryo production. Oocyte recovery results were T1: 33, T2: 38, and T3: 20 (p: 0.209). Oocyte classification by grade established the highest percentage in T1: 25.8%, T2: 19.3%, and T3: 13.7% (p: 0.323), all in grade I. Expansion (scale 1-4) was similar between T1 and T2 with 3.25 (p: 0.667). Maturation averages were T1 at 47.92%, T2 at 66.07%, and T3 at 70.01% (p: 0.615). The cleavage measurement results showed the following percentages T1: 50%, T2: 22%, and T3: 66.7%. In in vitro embryo production, T1 and T2 reached the morula stage. As a result, there were no differences between treatments, so testing different protocols and routes of FSH-p administration in further research is recommended.