Examinando por Autor "Chimbo Granda, Adrián Esteban"
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- ÍtemAcceso AbiertoCaracterización fenotípica y molecular de cepas de wickerhamomyces anomalus que colonizan la mucosa nasal de cuyes domésticos (cavia porcellus) en granjas de la pronvincia de Cañar (Ecuador)(Universidad Católica de Cuenca., 2023) Chimbo Granda, Adrián Esteban; Yarzábal Rodríguez, Andrés; 010581231-7INTRODUCTION: Zoonosis is a disease that has jumped from non-human animals to humans. Therefore, conducting research to identify possible zoonotic pathogens that may affect the population is essential. OBJECTIVE: This research aimed to characterize phenotypically and molecularly strains of Wickerhamomyces anomalous isolated from the nasal mucosa of guinea pigs raised in farms in the Cañar Province (Ecuador). MATERIALS Y METHODS: The methodology was based on conventional microbiology procedures and techniques. Different culture media, including Sabouraud Dextrose Agar, Papa Dextrose Agar, and HiChrome Candida, were used to evaluate the morphological and physiological characteristics of the strains studied. Enzyme activity tests were performed on media supplemented with sheep blood (hemolysis), egg yolk (phospholipase), and milk (caseinolysis). Penetration assays were performed in the SDA medium. VITEK®2 equipment was used to measure the susceptibility of the strains to antifungals. The molecular phylogeny of the strains was established with bioinformatics programs such as MEGA X, BLAST, and nucleotide sequences from GenBank. RESULTS: The most relevant results include the estimation of the optimal growth temperature (23 °C> opt. temp. <37 °C), the hemolytic activity, the ability to penetrate solid media of all isolates with the presence of true hyphae (activation of a phenotypic-dimorphism switching), and the evaluation of evolutionary associations with other strains of the same species (some clinically significant). The sum of these results suggests a potential health risk for the population